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Je něco špatně v tomto záznamu ?
Head and neck squamous cancer stromal fibroblasts produce growth factors influencing phenotype of normal human keratinocytes
H. Strnad, L. Lacina, M. Kolář, Z. Čada, Č. Vlček, B. Dvořánková, J. Betka, J. Plzák, M. Chovanec, J. Šáchová, J. Valach, M. Urbanová, K. Smetana
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
ProQuest Central
od 1997-01-01 do 2017-12-31
Medline Complete (EBSCOhost)
od 2000-01-01 do Před 1 rokem
Nursing & Allied Health Database (ProQuest)
od 1997-01-01 do 2017-12-31
Health & Medicine (ProQuest)
od 1997-01-01 do 2017-12-31
Public Health Database (ProQuest)
od 1997-01-01 do 2017-12-31
- MeSH
- buňky NIH 3T3 MeSH
- čipová analýza proteinů MeSH
- fenotyp MeSH
- fibroblasty metabolismus MeSH
- imunohistochemie MeSH
- insulinu podobný růstový faktor II biosyntéza MeSH
- keratinocyty cytologie metabolismus MeSH
- kostní morfogenetický protein 4 biosyntéza MeSH
- lidé MeSH
- myši MeSH
- nádory hlavy a krku metabolismus patologie MeSH
- rekombinantní proteiny biosyntéza MeSH
- spinocelulární karcinom metabolismus patologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Epithelial-mesenchymal interaction between stromal fibroblasts and cancer cells influences the functional properties of tumor epithelium, including the tumor progression and spread. We compared fibroblasts prepared from stroma of squamous cell carcinoma and normal dermal fibroblasts concerning their biological activity toward normal keratinocytes assessed by immunocytochemistry and profiling of gene activation for growth factors/cytokines by microarray chip technology. IGF-2 and BMP-4 were determined as candidate factors responsible for tumor-associated fibroblast activity that influences normal epithelia. This effect was confirmed by addition of recombinant IGF-2 and BMP4, respectively, to the culture medium. This hypothesis was also verified by inhibition experiments where blocking antibodies were employed in the medium conditioned by cancer-associated fibroblast. Presence of these growth factors was also detected in tumor samples.
Citace poskytuje Crossref.org
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- $a Epithelial-mesenchymal interaction between stromal fibroblasts and cancer cells influences the functional properties of tumor epithelium, including the tumor progression and spread. We compared fibroblasts prepared from stroma of squamous cell carcinoma and normal dermal fibroblasts concerning their biological activity toward normal keratinocytes assessed by immunocytochemistry and profiling of gene activation for growth factors/cytokines by microarray chip technology. IGF-2 and BMP-4 were determined as candidate factors responsible for tumor-associated fibroblast activity that influences normal epithelia. This effect was confirmed by addition of recombinant IGF-2 and BMP4, respectively, to the culture medium. This hypothesis was also verified by inhibition experiments where blocking antibodies were employed in the medium conditioned by cancer-associated fibroblast. Presence of these growth factors was also detected in tumor samples.
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