-
Je něco špatně v tomto záznamu ?
Flubendazole metabolism and biotransformation enzymes activities in healthy sheep and sheep with haemonchosis
H. Bártíková, V. Krízová, J. Lamka, V. Kubícek, L. Skálová, B. Szotáková,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu klinické zkoušky kontrolované, časopisecké články, práce podpořená grantem
- MeSH
- biotransformace MeSH
- Haemonchus účinky léků MeSH
- hemonchóza farmakoterapie metabolismus veterinární MeSH
- játra enzymologie metabolismus MeSH
- mebendazol analogy a deriváty metabolismus terapeutické užití MeSH
- nemoci ovcí metabolismus MeSH
- ovce MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- klinické zkoušky kontrolované MeSH
- práce podpořená grantem MeSH
The aim of this project was to study the influence of haemonchosis, a common parasitic infection of small ruminants caused by Haemonchus contortus, on the activity of biotransformation enzymes and on in vitro flubendazole (FLU) biotransformation in liver and small intestine of lambs (Ovis aries). Twelve lambs were divided into three groups: non-infected animals, animals orally infected with larvae of H. contortus ISE strain for 7 weeks and for 11 weeks. At the end of the experiment, hepatic and intestinal subcellular fractions were prepared and used for assays of biotransformation enzymes activities and FLU metabolism testing. The activities of hepatic cytochromes P450, flavine monooxygenases and carbonyl-reducing enzymes were decreased in infected animals. UDP-glucuronosyl transferase activity was significantly lower (by 35%) in 11 weeks infected animals than that in control animals. When in vitro metabolism of FLU was compared in control and infected animals, significantly lower velocity of FLU reduction was found in infected animals. Slower FLU reduction may be beneficial for the haemonchosis treatment using FLU, because FLU will remain longer in the organism and could cause longer contact of parasites with FLU.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc12025739
- 003
- CZ-PrNML
- 005
- 20121207122321.0
- 007
- ta
- 008
- 120817s2010 enk f 000 0#eng||
- 009
- AR
- 024 7_
- $a 10.1111/j.1365-2885.2009.01112.x $2 doi
- 035 __
- $a (PubMed)20444026
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Bártíková, H $u Faculty of Pharmacy, Charles University, Heyrovského, Hradec Králové, Czech Republic.
- 245 10
- $a Flubendazole metabolism and biotransformation enzymes activities in healthy sheep and sheep with haemonchosis / $c H. Bártíková, V. Krízová, J. Lamka, V. Kubícek, L. Skálová, B. Szotáková,
- 520 9_
- $a The aim of this project was to study the influence of haemonchosis, a common parasitic infection of small ruminants caused by Haemonchus contortus, on the activity of biotransformation enzymes and on in vitro flubendazole (FLU) biotransformation in liver and small intestine of lambs (Ovis aries). Twelve lambs were divided into three groups: non-infected animals, animals orally infected with larvae of H. contortus ISE strain for 7 weeks and for 11 weeks. At the end of the experiment, hepatic and intestinal subcellular fractions were prepared and used for assays of biotransformation enzymes activities and FLU metabolism testing. The activities of hepatic cytochromes P450, flavine monooxygenases and carbonyl-reducing enzymes were decreased in infected animals. UDP-glucuronosyl transferase activity was significantly lower (by 35%) in 11 weeks infected animals than that in control animals. When in vitro metabolism of FLU was compared in control and infected animals, significantly lower velocity of FLU reduction was found in infected animals. Slower FLU reduction may be beneficial for the haemonchosis treatment using FLU, because FLU will remain longer in the organism and could cause longer contact of parasites with FLU.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a biotransformace $7 D001711
- 650 _2
- $a hemonchóza $x farmakoterapie $x metabolismus $x veterinární $7 D006188
- 650 _2
- $a Haemonchus $x účinky léků $7 D006189
- 650 _2
- $a játra $x enzymologie $x metabolismus $7 D008099
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a mebendazol $x analogy a deriváty $x metabolismus $x terapeutické užití $7 D008463
- 650 _2
- $a ovce $7 D012756
- 650 _2
- $a nemoci ovcí $x metabolismus $7 D012757
- 655 _2
- $a klinické zkoušky kontrolované $7 D018848
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Krízová, V
- 700 1_
- $a Lamka, J
- 700 1_
- $a Kubícek, V
- 700 1_
- $a Skálová, L
- 700 1_
- $a Szotáková, B
- 773 0_
- $w MED00006035 $t Journal of veterinary pharmacology and therapeutics $x 1365-2885 $g Roč. 33, č. 1 (2010), s. 56-62
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/20444026 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y m
- 990 __
- $a 20120817 $b ABA008
- 991 __
- $a 20121207122355 $b ABA008
- 999 __
- $a ok $b bmc $g 947781 $s 783085
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2010 $b 33 $c 1 $d 56-62 $i 1365-2885 $m Journal of veterinary pharmacology and therapeutics $n J Vet Pharmacol Ther $x MED00006035
- LZP __
- $a Pubmed-20120817/10/03
