Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Oxidative stress may modify zinc protoporphyrin/heme ratio in hematofluorometry

SJ. Janousek, L. Rosa, S. Janousek, D. Jirova, K. Kejlova,

. 2010 ; 32 (1 Pt 2) : 40-49.

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc12026058

Grantová podpora
NS9802 MZ0 CEP - Centrální evidence projektů

Washed red blood cells (RBCs), supplemented or non-supplemented with sodium azide (to inhibit catalase activity), were exposed to different concentrations of hydrogen peroxide as well as ascorbic acid. Strikingly, catalase within RBCs protected the cells against exogenic hydrogen peroxide even at millimolar concentrations. However, the activity of the erythrocytic catalase failed to protect the RBCs when they were exposed to an oxidative burst of stimulated polymorphonuclear cells (PMNCs) in the presence of several reactive species in addition to peroxide. Oxyhemoglobin, with an excess of hydrogen peroxide, formed oxidized hemoglobin species and caused protein denaturation as well as the rise of heme degradation products which was suspected to falsify zinc protoporphyrin/heme (ZPP/heme) ratio as assessed by hematofluorometry. Our experiments may thus imply that the non-fluorescent hemoglobin background can be modified by reactive oxygen species (ROS) and this can lead to a spurious ZPP/heme ratio. We discuss this phenomenon with respect to ZPP quantification in clinical practice.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc12026058
003      
CZ-PrNML
005      
20141105111707.0
007      
ta
008      
120817s2010 enk f 000 0#eng||
009      
AR
024    7_
$a 10.1111/j.1751-553x.2008.01116.x $2 doi
035    __
$a (PubMed)19055648
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Janousek, S. J. $u National Institute of Public Health, Centre of Health and Living Conditions, Prague, Czech Republic. sjanousek@szu.cz
245    10
$a Oxidative stress may modify zinc protoporphyrin/heme ratio in hematofluorometry / $c SJ. Janousek, L. Rosa, S. Janousek, D. Jirova, K. Kejlova,
520    9_
$a Washed red blood cells (RBCs), supplemented or non-supplemented with sodium azide (to inhibit catalase activity), were exposed to different concentrations of hydrogen peroxide as well as ascorbic acid. Strikingly, catalase within RBCs protected the cells against exogenic hydrogen peroxide even at millimolar concentrations. However, the activity of the erythrocytic catalase failed to protect the RBCs when they were exposed to an oxidative burst of stimulated polymorphonuclear cells (PMNCs) in the presence of several reactive species in addition to peroxide. Oxyhemoglobin, with an excess of hydrogen peroxide, formed oxidized hemoglobin species and caused protein denaturation as well as the rise of heme degradation products which was suspected to falsify zinc protoporphyrin/heme (ZPP/heme) ratio as assessed by hematofluorometry. Our experiments may thus imply that the non-fluorescent hemoglobin background can be modified by reactive oxygen species (ROS) and this can lead to a spurious ZPP/heme ratio. We discuss this phenomenon with respect to ZPP quantification in clinical practice.
650    _2
$a erytrocyty $x chemie $7 D004912
650    _2
$a falešně pozitivní reakce $7 D005189
650    _2
$a fluorometrie $7 D005470
650    _2
$a hem $x analýza $x metabolismus $7 D006418
650    _2
$a lidé $7 D006801
650    _2
$a oxidační stres $7 D018384
650    _2
$a protoporfyriny $x krev $x metabolismus $7 D011524
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Rosa, L. $u Hematology Department, Kralovske Vinohrady Faculty Hospital, Prague, Czech Republic
700    1_
$a Janoušek, S. $u Cardiology Department of Medical Faculty, Brno, Czech Republic
700    1_
$a Jirova, D. $u National Institute of Public Health, Centre of Health and Living Conditions, Prague, Czech Republic
700    1_
$a Kejlova, K. $u National Institute of Public Health, Centre of Health and Living Conditions, Prague, Czech Republic
773    0_
$w MED00153487 $t International journal of laboratory hematology $x 1751-553X $g Roč. 32, č. 1 Pt 2 (2010), s. 40-49
856    41
$u https://pubmed.ncbi.nlm.nih.gov/19055648 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y m $z 0
990    __
$a 20120817 $b ABA008
991    __
$a 20141105111715 $b ABA008
999    __
$a ok $b bmc $g 948100 $s 783404
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2010 $b 32 $c 1 Pt 2 $d 40-49 $i 1751-553X $m International journal of laboratory hematology $n Int J Lab Hematol $x MED00153487
GRA    __
$a NS9802 $p MZ0
LZP    __
$a Pubmed-20120817/10/03

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace