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Study of oostatic peptide uptake and metabolism in developing ovaries of the flesh fly, Neobellieria bullata
B. Bennettová, J. Slaninová, V. Vlasáková, J. Hlavácek, J. Holík, R. Tykva,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Directory of Open Access Journals
od 2001
Free Medical Journals
od 2001
PubMed Central
od 2001
Europe PubMed Central
od 2001
Open Access Digital Library
od 2001-01-01 do 2015-12-31
Open Access Digital Library
od 2001-01-01
Open Access Digital Library
od 2001-01-01
Open Access Digital Library
od 2001-01-01
Oxford Journals Open Access Collection
od 2001-01-01
ROAD: Directory of Open Access Scholarly Resources
od 2001
PubMed
20572785
DOI
10.1673/031.010.4801
Knihovny.cz E-zdroje
- MeSH
- biologický transport MeSH
- Diptera metabolismus MeSH
- orgánové kultury - kultivační techniky MeSH
- ovarium růst a vývoj metabolismus MeSH
- peptidy metabolismus MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The uptake and metabolism of the oostatic pentapeptide analogue of trypsin modulating oostatic factor (TMOF), H-Tyr-Asp-Pro-Ala-Pro-OH (5P), in ovaries of Neobellieria bullata (Parker) (Diptera: Sarcophagidae) were analyzed during their developmental stages. During selected stages of yolk deposition, the fate of [3HPro(3)]5P after its in vivo injection was compared to its uptake after in vitro incubation of dissected ovaries. The ovaries were analyzed from 30 s to 180 min after incubation. A detection sensitivity of 60-100 fmol of the labeled 5P was achieved using radio-high performance liquid chromatography. While the uptake of the applied radioactivity strongly depended on the stage of vitellogenesis, especially for the in vitro experiment, degradation of 5P was very quick and independent of whether the label was injected or incubated with the ovaries, regardless of the developmental stage of ovaries. No tracers of 5P were detected at 30 s after applying the labeled 5P in all tests.
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