Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Automated scoring of lymphocyte micronuclei by the MetaSystems Metafer image cytometry system and its application in studies of human mutagen sensitivity and biodosimetry of genotoxin exposure

A. Rossnerova, M. Spatova, C. Schunck, R. J. Sram

. 2011 ; 26 (1) : 169-175.

Language English Country England, Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't, Review

Persistent link   https://www.medvik.cz/link/bmc12026590

Automated image analysis scoring of micronuclei (MN) in cells can facilitate the objective and rapid measurement of genetic damage in mammalian and human cells. This approach was repeatedly developed and tested over the past two decades but none of the systems were sufficiently robust for routine analysis of MN until recently. New methodological, hardware and software developments have now allowed more advanced systems to become available. This mini-review presents the current stage of development and validation of the Metasystems Metafer MNScore system for automated image analysis scoring of MN in cytokinesis-blocked binucleated lymphocytes, which is the best-established method for studying MN formation in humans. The results and experience of users of this system from 2004 until today are reviewed in this paper. Significant achievements in the application of this method in research related to mutagen sensitivity phenotype in cancer risk, radiation biodosimetry and biomonitoring studies of air pollution (enriched by new data) are described. Advantages as well as limitations of automated image analysis in comparison with traditional visual analysis are discussed. The current increased use of the Metasystems Metafer MNScore system in various studies and the growing number of publications based on automated image analysis scoring of MN is promising for the ongoing and future application of this approach.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc12026590
003      
CZ-PrNML
005      
20160228092846.0
007      
ta
008      
120816s2011 enk f 000 0#eng||
009      
AR
024    7_
$a 10.1093/mutage/geq057 $2 doi
035    __
$a (PubMed)21164199
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Rossnerova, Andrea $u Department of Genetic Ecotoxicology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic
245    10
$a Automated scoring of lymphocyte micronuclei by the MetaSystems Metafer image cytometry system and its application in studies of human mutagen sensitivity and biodosimetry of genotoxin exposure / $c A. Rossnerova, M. Spatova, C. Schunck, R. J. Sram
520    9_
$a Automated image analysis scoring of micronuclei (MN) in cells can facilitate the objective and rapid measurement of genetic damage in mammalian and human cells. This approach was repeatedly developed and tested over the past two decades but none of the systems were sufficiently robust for routine analysis of MN until recently. New methodological, hardware and software developments have now allowed more advanced systems to become available. This mini-review presents the current stage of development and validation of the Metasystems Metafer MNScore system for automated image analysis scoring of MN in cytokinesis-blocked binucleated lymphocytes, which is the best-established method for studying MN formation in humans. The results and experience of users of this system from 2004 until today are reviewed in this paper. Significant achievements in the application of this method in research related to mutagen sensitivity phenotype in cancer risk, radiation biodosimetry and biomonitoring studies of air pollution (enriched by new data) are described. Advantages as well as limitations of automated image analysis in comparison with traditional visual analysis are discussed. The current increased use of the Metasystems Metafer MNScore system in various studies and the growing number of publications based on automated image analysis scoring of MN is promising for the ongoing and future application of this approach.
650    _2
$a mladiství $7 D000293
650    _2
$a dospělí $7 D000328
650    _2
$a senioři $7 D000368
650    _2
$a dítě $7 D002648
650    _2
$a předškolní dítě $7 D002675
650    _2
$a vztah mezi dávkou a účinkem léčiva $7 D004305
650    _2
$a lidé $7 D006801
650    _2
$a obrazová cytometrie $x metody $7 D019044
650    _2
$a počítačové zpracování obrazu $x metody $7 D007091
650    _2
$a kojenec $7 D007223
650    _2
$a novorozenec $7 D007231
650    _2
$a počet lymfocytů $x metody $7 D018655
650    _2
$a lymfocyty $x účinky léků $x ultrastruktura $7 D008214
650    _2
$a mikrojádra chromozomálně defektní $7 D048629
650    _2
$a mikrojaderné testy $x přístrojové vybavení $7 D015162
650    _2
$a lidé středního věku $7 D008875
650    _2
$a mutageny $x toxicita $7 D009153
650    _2
$a mladý dospělý $7 D055815
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
655    _2
$a přehledy $7 D016454
700    1_
$a Špátová, Milada $7 xx0244281
700    1_
$a Schunck, Christian $u MetaSystems GmbH, Altlussheim, Ulm, Germany. cschunck@metasystems.de
700    1_
$a Šrám, Radim J., $d 1939- $7 jk01130963 $u Institute of Experimental Medicine, Academy of Sciences of the Czech Republic (ASCR), 142 20 Prague, Czech Republic
773    0_
$w MED00003429 $t Mutagenesis $x 1464-3804 $g Roč. 26, č. 1 (2011), s. 169-175
856    41
$u https://pubmed.ncbi.nlm.nih.gov/21164199 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y m $z 0
990    __
$a 20120816 $b ABA008
991    __
$a 20160228092856 $b ABA008
999    __
$a ok $b bmc $g 948632 $s 783936
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2011 $b 26 $c 1 $d 169-175 $i 1464-3804 $m Mutagenesis $n Mutagenesis $x MED00003429
LZP    __
$b NLK122 $a Pubmed-20120816/11/01
Back

Find record

Citation metrics

Archiving options