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Synthetic poly(amino acid) hydrogels with incorporated cell-adhesion peptides for tissue engineering
H. Studenovská, P. Vodicka, V. Proks, J. Hlucilová, J. Motlík, F. Rypácek
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20084624
DOI
10.1002/term.256
Knihovny.cz E-resources
- MeSH
- Actins metabolism MeSH
- Amino Acids pharmacology MeSH
- Biocompatible Materials chemistry pharmacology MeSH
- Cell Adhesion drug effects MeSH
- Fluorescence MeSH
- Hydrogels chemistry pharmacology MeSH
- Cells, Cultured MeSH
- Mesenchymal Stem Cells cytology metabolism drug effects MeSH
- Polymers chemistry pharmacology MeSH
- Porosity drug effects MeSH
- Surface Properties drug effects MeSH
- Proteins chemistry pharmacology MeSH
- Sus scrofa MeSH
- Tissue Engineering methods MeSH
- Tubulin metabolism MeSH
- Cell Shape drug effects MeSH
- Cell Survival drug effects MeSH
- Vinculin metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Preparation of soft poly(amino acid) hydrogels containing biomimetic cell-adhesive peptides was investigated. Covalently crosslinked gels were formed by radical co-polymerization of methacryloylated macromonomer poly[N(5)-(2-hydroxyethyl)-L-glutamine-stat-L-alanine-stat-methacryloyllysine] with 2-hydroxyethyl methacrylate (HEMA) as minor co-monomer. Hydrogels carrying biomimetic peptides were prepared by using methacryloylated peptides, such as methacryloyl-GGGRGDSG-OH and methacryloyl-GGGYIGSR-OH, as additional monomers in the polymerization mixture. Mechanical stability and swelling in water of the hydrogels obtained for different solid:water and polypeptide:HEMA ratios were evaluated. The microporosity of gels (5-20 microm), dependent on the polyHEMA phase separation in water, was followed by low-vacuum SEM. The effect of biomimetic modification of hydrogels with RGDS and YIGSR peptides on the seeding efficiency of porcine mesenchymal stem cells (MSCs) was studied in vitro. While unmodified hydrogels showed very low cell adhesion, due to their highly hydrophilic nature, the incorporation of adhesive peptides significantly improved the adhesion and viability of seeded cells.
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