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Determination of oxygen radical absorbance capacity of black cumin (Nigella sativa) seed quinone compounds

H. Tesarova, B. Svobodova, L. Kokoska, P. Marsik, M. Pribylova, P. Landa, J. Vadlejch

. 2011 ; 6 (2) : 213-216.

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc12027003

In this study, the antioxidant capacities of main quinone constituents of Nigella sativa seeds, namely dithymoquinone (1), thymohydroquinone (2) and thymoquinone (3), were compared using DPPH and ORAC methods. The best scavenging activity was produced by 2, which showed a remarkable activity of 2.60 Trolox equivalents (TE) in a concentration range between 1.6 and 6.4 microg/mL and IC50 value of 2.4 microg/mL in ORAC and DPPH assays, respectively. Contrastingly, 3 possessed only weak DPPH scavenging efficacy (IC50 = 170 microg/mL) but significant antioxidative action of 1.91 TE in ORAC assay. No effect has been observed for 1. Additionally, modified protocol for synthesis of 2 has been developed with aim to enhance its availability for further studies as well as for its future potential use. Based on the results of this study, we conclude that 2 could be considered as a compound with prospective antioxidative properties.

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$a Tesařová, Hana. $7 _AN025057 $u Department of Crop Sciences and Agroforestry, Institute of Tropics and Subtropics, Czech University of Life Sciences Prague, Kamycka 129, 165 21, Prague 6-Suchdol, Czech Republic
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$a Determination of oxygen radical absorbance capacity of black cumin (Nigella sativa) seed quinone compounds / $c H. Tesarova, B. Svobodova, L. Kokoska, P. Marsik, M. Pribylova, P. Landa, J. Vadlejch
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$a In this study, the antioxidant capacities of main quinone constituents of Nigella sativa seeds, namely dithymoquinone (1), thymohydroquinone (2) and thymoquinone (3), were compared using DPPH and ORAC methods. The best scavenging activity was produced by 2, which showed a remarkable activity of 2.60 Trolox equivalents (TE) in a concentration range between 1.6 and 6.4 microg/mL and IC50 value of 2.4 microg/mL in ORAC and DPPH assays, respectively. Contrastingly, 3 possessed only weak DPPH scavenging efficacy (IC50 = 170 microg/mL) but significant antioxidative action of 1.91 TE in ORAC assay. No effect has been observed for 1. Additionally, modified protocol for synthesis of 2 has been developed with aim to enhance its availability for further studies as well as for its future potential use. Based on the results of this study, we conclude that 2 could be considered as a compound with prospective antioxidative properties.
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$a Svobodová, Blanka $7 xx0116900 $u Institute of Biophysics, Medical University of Graz, A-8010 Graz, Austria
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$a Kokoška, Ladislav $7 pna2005280907 $u Department of Crop Sciences and Agroforestry, Institute of Tropics and Subtropics, Czech University of Life Sciences Prague, Kamycka 129, CZ-165 21 Prague 6-Suchdo
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$a Maršík, Petr $7 stk2008429102 $u Department of Plant Tissue Cultures, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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$a Kvasnicová, Marie $7 xx0100657 $u Laboratory of Plant Biotechnologies, Joint Laboratory of Institute of Experimental Botany AS CR, v.v.i. and Research Institute of Crop Production, v.v.i., Rozvojová 263, 165 02 Prague 6, Czech Republic
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$a Landa, Přemysl, $d 1978- $7 stk2008441541 $u Laboratory of Plant Biotechnologies, Institute of Experimental Botany AS CR, v.v.i., 165 02 Prague 6 - Lysolaje, Czech Republic. landa@ueb.cas.cz
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$a Vadlejch, Jaroslav. $7 _AN036244 $u Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, CZ-165 21 Prague 6-Suchdol
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