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Inhibition of SAH-hydrolase activity during seed germination leads to deregulation of flowering genes and altered flower morphology in tobacco

J. Fulneček, R. Matyášek, I. Votruba, A. Holý, K. Křížová, A. Kovařík

. 2011 ; 285 (3) : 225-236. [pub] 20110128

Jazyk angličtina Země Německo

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc12027091
E-zdroje Online Plný text

NLK ProQuest Central od 1997-03-01 do 2017-12-31
Medline Complete (EBSCOhost) od 2005-03-01 do Před 1 rokem
Health & Medicine (ProQuest) od 1997-03-01 do 2017-12-31

Odkazy

PubMed 21274566
DOI 10.1007/s00438-011-0601-8
Knihovny.cz E-zdroje

Developmental processes are closely connected to certain states of epigenetic information which, among others, rely on methylation of chromatin. S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) are key cofactors of enzymes catalyzing DNA and histone methylation. To study the consequences of altered SAH/SAM levels on plant development we applied 9-(S)-(2,3-dihydroxypropyl)-adenine (DHPA), an inhibitor of SAH-hydrolase, on tobacco seeds during a short phase of germination period (6 days). The transient drug treatment induced: (1) dosage-dependent global DNA hypomethylation mitotically transmitted to adult plants; (2) pleiotropic developmental defects including decreased apical dominance, altered leaf and flower symmetry, flower whorl malformations and reduced fertility; (3) dramatic upregulation of floral organ identity genes NTDEF, NTGLO and NAG1 in leaves. We conclude that temporal SAH-hydrolase inhibition deregulated floral genes expression probably via chromatin methylation changes. The data further show that plants might be particularly sensitive to accurate setting of SAH/SAM levels during critical developmental periods.

Citace poskytuje Crossref.org

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