• Je něco špatně v tomto záznamu ?

Cancer drug-resistance and a look at specific proteins: Rho GDP-dissociation inhibitor 2, Y-box binding protein 1, and HSP70/90 organizing protein in proteomics clinical application

H. Skalnikova, J. Martinkova, R. Hrabakova, P. Halada, M. Dziechciarkova, M. Hajduch, SJ. Gadher, A. Hammar, D. Enetoft, A. Ekefjard, O. Forsstrom-Olsson, H. Kovarova

. 2011 ; 10 (2) : 404-415.

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc12027294

Resistance to anti-cancer drugs is a well recognized problem and very often it is responsible for failure of the cancer treatment. In this study, the proteome alterations associated with the development of acquired resistance to cyclin-depedent kinases inhibitor bohemine, a promising anti-cancer drug, were analyzed with the primary aim of identifying potential targets of resistance within the cell that could pave a way to selective elimination of specific resistant cell types. A model of parental susceptible CEM T-lymphoblastic leukemia cells and its resistant counterpart CEM-BOH was used and advanced 2-D liquid chromatography was applied to fractionate cellular proteins. Differentially expressed identified proteins were further verified using immunoblotting and immunohistochemistry. Our study has revealed that Rho GDP-dissociation inhibitor 2, Y-box binding protein 1, and the HSP70/90 organizing protein have a critical role to play in resistance to cyclin-depedent kinases inhibitor. The results indicated not only that quantitative protein changes play an important role in drug-resistance, but also that there are various other parameters such as truncation, post-translational modification(s), and subcellular localization of selected proteins. Furthermore, these proteins were validated for their roles in drug resistance using different cell lines resistant to diverse representatives of anti-cancer drugs such as vincristine and daunorubicin.

000      
00000naa a2200000 a 4500
001      
bmc12027294
003      
CZ-PrNML
005      
20160318160530.0
007      
ta
008      
120816s2011 xxu f 000 0#eng||
009      
AR
024    7_
$a 10.1021/pr100468w $2 doi
035    __
$a (PubMed)21067243
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Skalníková, Helena $7 xx0119428 $u Institute of Animal Physiology and Genetics AS CR, vvi, Libechov, Czech Republic
245    10
$a Cancer drug-resistance and a look at specific proteins: Rho GDP-dissociation inhibitor 2, Y-box binding protein 1, and HSP70/90 organizing protein in proteomics clinical application / $c H. Skalnikova, J. Martinkova, R. Hrabakova, P. Halada, M. Dziechciarkova, M. Hajduch, SJ. Gadher, A. Hammar, D. Enetoft, A. Ekefjard, O. Forsstrom-Olsson, H. Kovarova
520    9_
$a Resistance to anti-cancer drugs is a well recognized problem and very often it is responsible for failure of the cancer treatment. In this study, the proteome alterations associated with the development of acquired resistance to cyclin-depedent kinases inhibitor bohemine, a promising anti-cancer drug, were analyzed with the primary aim of identifying potential targets of resistance within the cell that could pave a way to selective elimination of specific resistant cell types. A model of parental susceptible CEM T-lymphoblastic leukemia cells and its resistant counterpart CEM-BOH was used and advanced 2-D liquid chromatography was applied to fractionate cellular proteins. Differentially expressed identified proteins were further verified using immunoblotting and immunohistochemistry. Our study has revealed that Rho GDP-dissociation inhibitor 2, Y-box binding protein 1, and the HSP70/90 organizing protein have a critical role to play in resistance to cyclin-depedent kinases inhibitor. The results indicated not only that quantitative protein changes play an important role in drug-resistance, but also that there are various other parameters such as truncation, post-translational modification(s), and subcellular localization of selected proteins. Furthermore, these proteins were validated for their roles in drug resistance using different cell lines resistant to diverse representatives of anti-cancer drugs such as vincristine and daunorubicin.
650    _2
$a antitumorózní látky $x farmakologie $7 D000970
650    _2
$a nádorové buněčné linie $7 D045744
650    _2
$a vysokoúčinná kapalinová chromatografie $7 D002851
650    _2
$a výpočetní biologie $7 D019295
650    _2
$a DNA vazebné proteiny $x metabolismus $7 D004268
650    _2
$a chemorezistence $7 D019008
650    _2
$a inhibitory disociace guaninnukleotidů $x metabolismus $7 D020730
650    _2
$a proteiny tepelného šoku $x metabolismus $7 D006360
650    _2
$a lidé $7 D006801
650    _2
$a imunoblotting $7 D015151
650    _2
$a biologické modely $7 D008954
650    _2
$a jaderné proteiny $x metabolismus $7 D009687
650    _2
$a mapování interakce mezi proteiny $7 D025941
650    _2
$a proteom $x analýza $x metabolismus $7 D020543
650    _2
$a puriny $x farmakologie $7 D011687
650    _2
$a spektrofotometrie ultrafialová $7 D013056
650    _2
$a subcelulární frakce $x metabolismus $7 D013347
650    _2
$a nádorové supresorové proteiny $x metabolismus $7 D025521
650    _2
$a protein 1 vázající Y-box $7 D051840
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Martínková, Jiřina, $d 1940- $u Institute of Animal Physiology and Genetics AS CR, v.v.i., Rumburska 89, 277 21 Libechov, Czech Republic $7 nlk19990073537
700    1_
$a Hrabakova, Rita $u Institute of Animal Physiology and Genetics AS CR, v.v.i., Rumburska 89, 277 21 Libechov, Czech Republic
700    1_
$a Halada, Petr, $d 1972- $7 xx0063115 $u Institute of Microbiology AS CR, v.v.i., Videnska 1083, 142 20 Prague, Czech Republic
700    1_
$a Dziechciarková, Marta $7 xx0080459
700    1_
$a Hajdúch, Marián $7 xx0050218 $u Laboratory of Experimental Medicine, Institute of Translational and Molecular Medicine, Faculty of Medicine and Dentistry, Palacky University and University Hospital, Puskinova 6, 775 20 Olomouc, Czech Republic
700    1_
$a Gadher, Suresh Jivan $u Millipore Bioscience, 15 Research Park Drive, St. Charles, Missouri 63304, United States
700    1_
$a Hammar, Andreas $u Ludesi, Engelbrektsgatan 15, SE-211 33 Malmö, Sweden
700    1_
$a Enetoft, Daniel $u Ludesi, Engelbrektsgatan 15, SE-211 33 Malmö, Sweden
700    1_
$a Ekefjard, Andreas $u Ludesi, Engelbrektsgatan 15, SE-211 33 Malmö, Sweden
700    1_
$a Forsstrom-Olsson, Ola $u Ludesi, Engelbrektsgatan 15, SE-211 33 Malmö, Sweden
700    1_
$a Kovářová, Hana $7 xx0118797 $u Institute of Animal Physiology and Genetics AS CR, v.v.i., Rumburska 89, 277 21 Libechov, Czech Republic
773    0_
$w MED00166892 $t Journal of proteome research $x 1535-3907 $g Roč. 10, č. 2 (2011), s. 404-415
856    41
$u https://pubmed.ncbi.nlm.nih.gov/21067243 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y m $z 0
990    __
$a 20120816 $b ABA008
991    __
$a 20160318160248 $b ABA008
999    __
$a ok $b bmc $g 949336 $s 784640
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2011 $b 10 $c 2 $d 404-415 $i 1535-3907 $m Journal of proteome research $n J Proteome Res $x MED00166892
LZP    __
$b NLK112 $a Pubmed-20120816/11/02

Najít záznam

Citační ukazatele

Možnosti archivace