• Je něco špatně v tomto záznamu ?

Cytokeratin 8 is expressed in human corneoconjunctival epithelium, particularly in limbal epithelial cells

S. Merjava, K. Brejchova, A. Vernon, JT. Daniels, K. Jirsova

. 2011 ; 52 (2) : 787-794. [pub] 20110209

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc12027334

PURPOSE: The purpose of this study was to investigate the expression of cytokeratin (CK) 8 in the corneoconjunctival epithelium. METHODS: In 17 cadaveric corneoscleral discs and 3 other discs, the presence of CK8 alone or CK8, together with CK3, CK15, vimentin, and integrin α6, was investigated by using indirect immunohistochemistry on radial cryosections. Four corneoscleral discs stored in organ culture were used for the preparation of tangential sections of the limbus and for the isolation of limbal epithelial cells and their subsequent cultivation. CK8 expression was examined by RT-PCR in the corneal, limbal, and conjunctival epithelium. RESULTS: Sixty percent of the cadaveric corneoscleral samples and all samples stored in organ culture revealed positivity for CK8 in the basal epithelial layer of the limbus. Positive basal cells formed a single line or separated clusters. The signal for CK8 became weaker toward the surface of the limbal epithelium. The colocalization of CK8 with vimentin and CK15 in the limbus was also found. CK3 showed only occasional positivity in some of the surface limbal cells. The expression of integrin α6 in the basal membrane was absent or decreased under the CK8-positive clusters. Cell cultures revealed strong positivity for CK8 in approximately 80% of the cultured cells, and CK8 expression in the cornea, limbus, and conjunctiva was determined by RT-PCR. CONCLUSIONS: The study demonstrates the strong expression of CK8 in limbal epithelial basal cells, which is maintained during the differentiation and migration of the limbal cells toward the central corneal epithelium.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc12027334
003      
CZ-PrNML
005      
20160303101900.0
007      
ta
008      
120816s2011 xxu f 000 0#eng||
009      
AR
024    7_
$a 10.1167/iovs.10-5489 $2 doi
035    __
$a (PubMed)20926822
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Merjava, Stanislava $u Laboratory of the Biology and Pathology of the Eye, Institute of Inherited Metabolic Disorders, General Teaching Hospital and First Faculty of Medicine, Charles University, U nemocnice 2, 128 08, Prague, Czech Republic. merjava@centrum.cz
245    10
$a Cytokeratin 8 is expressed in human corneoconjunctival epithelium, particularly in limbal epithelial cells / $c S. Merjava, K. Brejchova, A. Vernon, JT. Daniels, K. Jirsova
520    9_
$a PURPOSE: The purpose of this study was to investigate the expression of cytokeratin (CK) 8 in the corneoconjunctival epithelium. METHODS: In 17 cadaveric corneoscleral discs and 3 other discs, the presence of CK8 alone or CK8, together with CK3, CK15, vimentin, and integrin α6, was investigated by using indirect immunohistochemistry on radial cryosections. Four corneoscleral discs stored in organ culture were used for the preparation of tangential sections of the limbus and for the isolation of limbal epithelial cells and their subsequent cultivation. CK8 expression was examined by RT-PCR in the corneal, limbal, and conjunctival epithelium. RESULTS: Sixty percent of the cadaveric corneoscleral samples and all samples stored in organ culture revealed positivity for CK8 in the basal epithelial layer of the limbus. Positive basal cells formed a single line or separated clusters. The signal for CK8 became weaker toward the surface of the limbal epithelium. The colocalization of CK8 with vimentin and CK15 in the limbus was also found. CK3 showed only occasional positivity in some of the surface limbal cells. The expression of integrin α6 in the basal membrane was absent or decreased under the CK8-positive clusters. Cell cultures revealed strong positivity for CK8 in approximately 80% of the cultured cells, and CK8 expression in the cornea, limbus, and conjunctiva was determined by RT-PCR. CONCLUSIONS: The study demonstrates the strong expression of CK8 in limbal epithelial basal cells, which is maintained during the differentiation and migration of the limbal cells toward the central corneal epithelium.
650    _2
$a mladiství $7 D000293
650    _2
$a dospělí $7 D000328
650    _2
$a senioři $7 D000368
650    _2
$a senioři nad 80 let $7 D000369
650    _2
$a buněčné kultury $7 D018929
650    _2
$a konjunktiva $x cytologie $x metabolismus $7 D003228
650    _2
$a epitelové buňky $x metabolismus $7 D004847
650    _2
$a rohovkový epitel $x cytologie $x metabolismus $7 D019573
650    _2
$a fluorescenční protilátková technika nepřímá $7 D019084
650    _2
$a exprese genu $7 D015870
650    _2
$a lidé $7 D006801
650    _2
$a integrin alfa6 $x metabolismus $7 D039503
650    _2
$a keratin-15 $x metabolismus $7 D053569
650    _2
$a keratin-3 $x metabolismus $7 D053558
650    _2
$a keratin-8 $x genetika $x metabolismus $7 D053533
650    _2
$a limbus corneae $x cytologie $x metabolismus $7 D016850
650    _2
$a fluorescenční mikroskopie $7 D008856
650    _2
$a lidé středního věku $7 D008875
650    _2
$a orgánové kultury - kultivační techniky $7 D009924
650    _2
$a messenger RNA $x genetika $7 D012333
650    _2
$a polymerázová řetězová reakce s reverzní transkripcí $7 D020133
650    _2
$a vimentin $x metabolismus $7 D014746
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Brejchová, Kristýna $7 _AN067034 $u Laboratory of the Biology and Pathology of the Eye, Institute of Inherited Metabolic Disorders, General Teaching Hospital and Charles University in Prague, 1st Faculty of Medicine, Prague, Czech Republic
700    1_
$a Vernon, Amanda $u Cells for Sight Transplantation and Research Programme, Department of Ocular Biology and Therapeutics, University College London (UCL) Institute of Ophthalmology, London, United Kingdom
700    1_
$a Daniels, Julie T. $u Cells for Sight Transplantation and Research Programme, Department of Ocular Biology and Therapeutics, University College London (UCL) Institute of Ophthalmology, London, United Kingdom.
700    1_
$a Jirsová, Kateřina, $d 1966- $7 xx0101780 $u Laboratory of the Biology and Pathology of the Eye, Institute of Inherited Metabolic Disorders; Ocular Tissue Bank, General Teaching Hospital and First Faculty of Medicine, Charles University in Prague, Czech Republic
773    0_
$w MED00002404 $t Investigative ophthalmology & visual science $x 1552-5783 $g Roč. 52, č. 2 (2011), s. 787-794
856    41
$u https://pubmed.ncbi.nlm.nih.gov/20926822 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y m $z 0
990    __
$a 20120816 $b ABA008
991    __
$a 20160303101616 $b ABA008
999    __
$a ok $b bmc $g 949376 $s 784680
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2011 $b 52 $c 2 $d 787-794 $e 20110209 $i 1552-5783 $m Investigative ophthalmology & visual science $n Invest Ophthalmol Vis Sci $x MED00002404
LZP    __
$b NLK112 $a Pubmed-20120816/11/02

Najít záznam

Citační ukazatele

Možnosti archivace