• Je něco špatně v tomto záznamu ?

Spectral analysis of doxorubicin accumulation and the indirect quantification of its DNA intercalation

O. Hovorka, V. Subr, D. Větvička, L. Kovář, J. Strohalm, M. Strohalm, A. Benda, M. Hof, K. Ulbrich, B. Ríhová

. 2010 ; 76 (3) : 514-524. [pub] 20100716

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc12027395

There is a wide range of techniques utilizing fluorescence of doxorubicin (Dox) commonly used for analysis of intracellular accumulation and destiny of various drug delivery systems containing this anthracycline antibiotic. Unfortunately, results of these studies can be significantly influenced by doxorubicin degradation product, 7,8-dehydro-9,10-desacetyldoxorubicinone (D*) forming spontaneously in aqueous environment, whose fluorescence strongly interfere with that of doxorubicin. Here, we define two microscopy techniques enabling to distinguish and separate Dox and D* emission based either on its spectral properties or on fluorescence lifetime analysis. To analyze influx and nuclear accumulation of Dox (free or polymer-bound) by flow cytometry, we propose using an indirect method based on its DNA intercalation competition with Hoechst 33342 rather than a direct measurement of doxorubicin fluorescence inside the cells.

000      
00000naa a2200000 a 4500
001      
bmc12027395
003      
CZ-PrNML
005      
20170621134914.0
007      
ta
008      
120816s2010 ne f 000 0#eng||
009      
AR
024    7_
$a 10.1016/j.ejpb.2010.07.008 $2 doi
035    __
$a (PubMed)20638475
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Hovorka, Ondřej, $u Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic. hovorka@biomed.cas.cz $d 1971- $7 xx0062705
245    10
$a Spectral analysis of doxorubicin accumulation and the indirect quantification of its DNA intercalation / $c O. Hovorka, V. Subr, D. Větvička, L. Kovář, J. Strohalm, M. Strohalm, A. Benda, M. Hof, K. Ulbrich, B. Ríhová
520    9_
$a There is a wide range of techniques utilizing fluorescence of doxorubicin (Dox) commonly used for analysis of intracellular accumulation and destiny of various drug delivery systems containing this anthracycline antibiotic. Unfortunately, results of these studies can be significantly influenced by doxorubicin degradation product, 7,8-dehydro-9,10-desacetyldoxorubicinone (D*) forming spontaneously in aqueous environment, whose fluorescence strongly interfere with that of doxorubicin. Here, we define two microscopy techniques enabling to distinguish and separate Dox and D* emission based either on its spectral properties or on fluorescence lifetime analysis. To analyze influx and nuclear accumulation of Dox (free or polymer-bound) by flow cytometry, we propose using an indirect method based on its DNA intercalation competition with Hoechst 33342 rather than a direct measurement of doxorubicin fluorescence inside the cells.
650    _2
$a buňky 3T3 $7 D016475
650    _2
$a zvířata $7 D000818
650    _2
$a antibiotika antitumorózní $x metabolismus $x farmakologie $7 D000903
650    _2
$a buněčné jádro $x metabolismus $7 D002467
650    _2
$a DNA $x metabolismus $7 D004247
650    _2
$a doxorubicin $x analogy a deriváty $x metabolismus $x farmakologie $7 D004317
650    _2
$a systémy cílené aplikace léků $7 D016503
650    _2
$a průtoková cytometrie $7 D005434
650    _2
$a lidé $7 D006801
650    _2
$a hydrofobní a hydrofilní interakce $7 D057927
650    _2
$a lymfom T-buněčný $x farmakoterapie $x metabolismus $7 D016399
650    _2
$a myši $7 D051379
650    _2
$a polymery $x metabolismus $7 D011108
650    _2
$a fluorescenční spektrometrie $7 D013050
650    _2
$a nádorové buňky kultivované $7 D014407
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Šubr, Vladimír, $d 1957- $7 xx0088449 $u Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
700    1_
$a Větvička, David $7 xx0214483 $u Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
700    1_
$a Kovář, Lubomír $7 xx0126758 $u Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
700    1_
$a Strohalm, Jiří $7 xx0109134 $u Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
700    1_
$a Strohalm, Martin $7 xx0143548 $u Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
700    1_
$a Benda, Aleš $7 xx0228989 $u J. Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
700    1_
$a Hof, Martin, $d 1962- $7 ntka172581 $u J. Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
700    1_
$a Ulbrich, Karel, $d 1947- $7 jo2004259877 $u Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
700    1_
$a Říhová, Blanka, $d 1942- $7 jo20000073671 $u Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
773    0_
$w MED00001640 $t European journal of pharmaceutics and biopharmaceutics official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V $x 1873-3441 $g Roč. 76, č. 3 (2010), s. 514-524
856    41
$u https://pubmed.ncbi.nlm.nih.gov/20638475 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y m $z 0
990    __
$a 20120816 $b ABA008
991    __
$a 20170621135333 $b ABA008
999    __
$a ok $b bmc $g 949437 $s 784741
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2010 $b 76 $c 3 $d 514-524 $e 20100716 $i 1873-3441 $m European journal of pharmaceutics and biopharmaceutics $n Eur J Pharm Biopharm $x MED00001640
LZP    __
$b NLK112 $a Pubmed-20120816/11/02

Najít záznam

Citační ukazatele

Možnosti archivace