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The problems of proteinuria measurement in urine with presence of Bence Jones protein
V. Maisnar, M. Tichy, J. Stulik, J. Vavrova, B. Friedecky, V. Palicka, J. Spirkova, L. Zaloudkova, L. Hernychova, J. Spacilova, T. Buchler, R. Hajek
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
NS10406
MZ0
CEP - Centrální evidence projektů
- MeSH
- 2D gelová elektroforéza MeSH
- Bence-Jonesova bílkovina moč MeSH
- lidé MeSH
- proteinurie moč MeSH
- reprodukovatelnost výsledků MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
OBJECTIVES: Protein concentration measurement in the urine can be problematic in the presence of Bence Jones protein. We have carried out an external quality control assessment with the participation of 79 clinical biochemistry laboratories from the Czech Republic and Slovakia. DESIGN AND METHODS: The laboratories received a reference urine sample obtained from a patient with multiple myeloma and lambda free light chain proteinuria and were asked to type the paraprotein using immunofixation and to measure total urinary protein using their established method, most commonly turbidimetry, pyrogallol red assay, and biuret assay. RESULTS: There was a very wide inter-laboratory variability in the protein concentration readouts with up to three-fold difference in some cases. High-resolution two-dimensional electrophoresis and linear mass spectrometry showed that a high proportion of the urinary paraprotein was composed of lambda light chain fragments with molecular weight of 12kDa. CONCLUSIONS: Our results highlight the challenges of reliable and reproducible measurement of urinary protein concentration in the presence of Bence Jones protein.
Citace poskytuje Crossref.org
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- $a Maisnar, Vladimír $7 xx0062937 $u 2nd Department of Internal Medicine-Division of Clinical Hematology, Charles University Faculty of Medicine and University Hospital, Sokolska 581, 500 05 Hradec Kralove, Czech Republic. maisnar@fnhk.cz
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- $a The problems of proteinuria measurement in urine with presence of Bence Jones protein / $c V. Maisnar, M. Tichy, J. Stulik, J. Vavrova, B. Friedecky, V. Palicka, J. Spirkova, L. Zaloudkova, L. Hernychova, J. Spacilova, T. Buchler, R. Hajek
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- $a OBJECTIVES: Protein concentration measurement in the urine can be problematic in the presence of Bence Jones protein. We have carried out an external quality control assessment with the participation of 79 clinical biochemistry laboratories from the Czech Republic and Slovakia. DESIGN AND METHODS: The laboratories received a reference urine sample obtained from a patient with multiple myeloma and lambda free light chain proteinuria and were asked to type the paraprotein using immunofixation and to measure total urinary protein using their established method, most commonly turbidimetry, pyrogallol red assay, and biuret assay. RESULTS: There was a very wide inter-laboratory variability in the protein concentration readouts with up to three-fold difference in some cases. High-resolution two-dimensional electrophoresis and linear mass spectrometry showed that a high proportion of the urinary paraprotein was composed of lambda light chain fragments with molecular weight of 12kDa. CONCLUSIONS: Our results highlight the challenges of reliable and reproducible measurement of urinary protein concentration in the presence of Bence Jones protein.
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