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Macrophage gene expression is related to obesity and the metabolic syndrome in human subcutaneous fat as well as in visceral fat
E. Klimcakova, B. Roussel, Z. Kovacova, M. Kovacikova, M. Siklova-Vitkova, M. Combes, J. Hejnova, P. Decaunes, J. J. Maoret, T. Vedral, N. Viguerie, V. Bourlier, A. Bouloumié, V. Stich, D. Langin
Language English Country Germany
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NS10519
MZ0
CEP Register
Digital library NLK
Full text - Article
Source
NLK
SpringerLink Journals
from 1997-01-01 to 2012-08-31
ProQuest Central
from 1999-01-01 to 1 year ago
Medline Complete (EBSCOhost)
from 2000-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 1999-01-01 to 1 year ago
Public Health Database (ProQuest)
from 1999-01-01 to 1 year ago
- MeSH
- Adult MeSH
- Cells, Cultured MeSH
- Middle Aged MeSH
- Humans MeSH
- Macrophages metabolism MeSH
- Metabolic Syndrome metabolism MeSH
- Young Adult MeSH
- Overweight metabolism MeSH
- Intra-Abdominal Fat cytology metabolism MeSH
- Obesity metabolism MeSH
- Subcutaneous Fat cytology metabolism MeSH
- Aged MeSH
- Adipose Tissue cytology metabolism MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Young Adult MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
AIMS/HYPOTHESIS: Our goal was to identify a set of human adipose tissue macrophage (ATM)-specific markers and investigate whether their gene expression in subcutaneous adipose tissue (SAT) as well as in visceral adipose tissue (VAT) is related to obesity and to the occurrence of the metabolic syndrome. METHODS: ATM-specific markers were identified by DNA microarray analysis of adipose tissue cell types isolated from SAT of lean and obese individuals. We then analysed gene expression of these markers by reverse transcription quantitative PCR in paired samples of SAT and VAT from 53 women stratified into four groups (lean, overweight, obese and obese with the metabolic syndrome). Anthropometric measurements, euglycaemic-hyperinsulinaemic clamp, blood analysis and computed tomography scans were performed. RESULTS: A panel of 24 genes was selected as ATM-specific markers based on overexpression in ATM compared with other adipose tissue cell types. In SAT and VAT, gene expression of ATM markers was lowest in lean and highest in the metabolic syndrome group. mRNA levels in the two fat depots were negatively correlated with glucose disposal rate and positively associated with indices of adiposity and the metabolic syndrome. CONCLUSIONS/INTERPRETATION: In humans, expression of ATM-specific genes increases with the degree of adiposity and correlates with markers of insulin resistance and the metabolic syndrome to a similar degree in SAT and in VAT.
Biochemistry Laboratory Biology Institute of Purpan CHU de Toulouse Toulouse France
Department of Surgery University Hospital Kralovské Vinohrady Prague Czech Republic
References provided by Crossref.org
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- $a AIMS/HYPOTHESIS: Our goal was to identify a set of human adipose tissue macrophage (ATM)-specific markers and investigate whether their gene expression in subcutaneous adipose tissue (SAT) as well as in visceral adipose tissue (VAT) is related to obesity and to the occurrence of the metabolic syndrome. METHODS: ATM-specific markers were identified by DNA microarray analysis of adipose tissue cell types isolated from SAT of lean and obese individuals. We then analysed gene expression of these markers by reverse transcription quantitative PCR in paired samples of SAT and VAT from 53 women stratified into four groups (lean, overweight, obese and obese with the metabolic syndrome). Anthropometric measurements, euglycaemic-hyperinsulinaemic clamp, blood analysis and computed tomography scans were performed. RESULTS: A panel of 24 genes was selected as ATM-specific markers based on overexpression in ATM compared with other adipose tissue cell types. In SAT and VAT, gene expression of ATM markers was lowest in lean and highest in the metabolic syndrome group. mRNA levels in the two fat depots were negatively correlated with glucose disposal rate and positively associated with indices of adiposity and the metabolic syndrome. CONCLUSIONS/INTERPRETATION: In humans, expression of ATM-specific genes increases with the degree of adiposity and correlates with markers of insulin resistance and the metabolic syndrome to a similar degree in SAT and in VAT.
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