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NHR-23 dependent collagen and hedgehog-related genes required for molting
NA. Kouns, J. Nakielna, F. Behensky, MW. Krause, Z. Kostrouch, M. Kostrouchova,
Language English Country United States
Document type Journal Article, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't
- MeSH
- Caenorhabditis elegans genetics growth & development MeSH
- Gene Deletion MeSH
- Molecular Sequence Data MeSH
- Caenorhabditis elegans Proteins genetics physiology MeSH
- Hedgehog Proteins metabolism MeSH
- Receptors, Cytoplasmic and Nuclear genetics physiology MeSH
- Amino Acid Sequence MeSH
- Molting genetics MeSH
- Transcription Factors genetics physiology MeSH
- Gene Expression Regulation, Developmental MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, N.I.H., Intramural MeSH
NHR-23, a conserved member of the nuclear receptor family of transcription factors, is required for normal development in Caenorhabditis elegans where it plays a critical role in growth and molting. In a search for NHR-23 dependent genes, we performed whole genome comparative expression microarrays on both control and nhr-23 inhibited synchronized larvae. Genes that decreased in response to nhr-23 RNAi included several collagen genes. Unexpectedly, several hedgehog-related genes were also down-regulated after nhr-23 RNAi. A homozygous nhr-23 deletion allele was used to confirm the RNAi knockdown phenotypes and the changes in gene expression. Our results indicate that NHR-23 is a critical co-regulator of functionally linked genes involved in growth and molting and reveal evolutionary parallels among the ecdysozoa.
References provided by Crossref.org
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