-
Something wrong with this record ?
Retron Se72 utilizes a unique strategy of the self-priming initiation of reverse transcription
L. Pilousova, I. Rychlik,
Language English Country Switzerland
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
PubMed Central
from 1997
ProQuest Central
from 1997-01-01 to 1 year ago
Medline Complete (EBSCOhost)
from 2000-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 1997-01-01 to 1 year ago
- MeSH
- 5' Untranslated Regions MeSH
- Bacterial Proteins genetics metabolism MeSH
- RNA, Bacterial genetics metabolism MeSH
- DNA, Bacterial biosynthesis MeSH
- DNA Primers chemistry metabolism MeSH
- Nucleic Acid Conformation MeSH
- RNA, Messenger chemistry metabolism MeSH
- Molecular Sequence Data MeSH
- Polymerase Chain Reaction MeSH
- RNA-Directed DNA Polymerase genetics metabolism MeSH
- Ribonuclease H metabolism physiology MeSH
- Salmonella enzymology MeSH
- Base Sequence MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Unlike all of the other retrons, the bacterial retron reverse transcriptase RrtE is capable of synthesizing small double-stranded DNA (sdsDNA) from template RNA. In this study, we analyzed the biosynthesis of the sdsDNA by RrtE in detail. We found out that the initiation of reverse transcription was dependent on a novel self-priming mechanism utilizing a free 3'OH of RNA that is reverse-transcribed into sdsDNA. The priming of the sdsDNA synthesis was not dependent on any particular nucleotide being used as a donor of 3'OH (unlike all of the other retrons, which prime from 2'OH of a particular guanosine) or any particular nucleotide being introduced into the sdsDNA first. Due to the relaxed demands for the initiation of reverse transcription, RrtE has the potential to generate dsDNA from different RNA transcripts in vivo.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc12028393
- 003
- CZ-PrNML
- 005
- 20121206124207.0
- 007
- ta
- 008
- 120817e20110331sz f 000 0#eng||
- 009
- AR
- 024 7_
- $a 10.1007/s00018-011-0671-0 $2 doi
- 035 __
- $a (PubMed)21452087
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a sz
- 100 1_
- $a Pilousova, Lenka $u Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic. pilousova@vri.cz
- 245 10
- $a Retron Se72 utilizes a unique strategy of the self-priming initiation of reverse transcription / $c L. Pilousova, I. Rychlik,
- 520 9_
- $a Unlike all of the other retrons, the bacterial retron reverse transcriptase RrtE is capable of synthesizing small double-stranded DNA (sdsDNA) from template RNA. In this study, we analyzed the biosynthesis of the sdsDNA by RrtE in detail. We found out that the initiation of reverse transcription was dependent on a novel self-priming mechanism utilizing a free 3'OH of RNA that is reverse-transcribed into sdsDNA. The priming of the sdsDNA synthesis was not dependent on any particular nucleotide being used as a donor of 3'OH (unlike all of the other retrons, which prime from 2'OH of a particular guanosine) or any particular nucleotide being introduced into the sdsDNA first. Due to the relaxed demands for the initiation of reverse transcription, RrtE has the potential to generate dsDNA from different RNA transcripts in vivo.
- 650 _2
- $a 5' nepřekládaná oblast $7 D020121
- 650 _2
- $a bakteriální proteiny $x genetika $x metabolismus $7 D001426
- 650 _2
- $a sekvence nukleotidů $7 D001483
- 650 _2
- $a DNA primery $x chemie $x metabolismus $7 D017931
- 650 _2
- $a DNA bakterií $x biosyntéza $7 D004269
- 650 _2
- $a molekulární sekvence - údaje $7 D008969
- 650 _2
- $a konformace nukleové kyseliny $7 D009690
- 650 _2
- $a polymerázová řetězová reakce $7 D016133
- 650 _2
- $a bakteriální RNA $x genetika $x metabolismus $7 D012329
- 650 _2
- $a messenger RNA $x chemie $x metabolismus $7 D012333
- 650 _2
- $a reverzní transkriptasa $x genetika $x metabolismus $7 D012194
- 650 _2
- $a ribonukleasa H $x metabolismus $x fyziologie $7 D016914
- 650 _2
- $a Salmonella $x enzymologie $7 D012475
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Rychlik, Ivan
- 773 0_
- $w MED00001078 $t Cellular and molecular life sciences : CMLS $x 1420-9071 $g Roč. 68, č. 21 (20110331), s. 3607-17
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/21452087 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y m
- 990 __
- $a 20120817 $b ABA008
- 991 __
- $a 20121206124241 $b ABA008
- 999 __
- $a ok $b bmc $g 950435 $s 785739
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2011 $b 68 $c 21 $d 3607-17 $e 20110331 $i 1420-9071 $m Cellular and molecular life sciences $n Cell Mol Life Sci $x MED00001078
- LZP __
- $a Pubmed-20120817/11/04
