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In vitro activation of CMV-specific human CD8(+) T cells by adenylate cyclase toxoids delivering pp65 epitopes
J. Jelinek, I. Adkins, Z. Mikulkova, J. Jagosova, R. Pacasova, S. Michlickova, P. Sebo, J. Michalek
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
NS9871
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Článek
Zdroj
NLK
Free Medical Journals
od 1997 do Před 1 rokem
Freely Accessible Science Journals
od 1997 do Před 1 rokem
ProQuest Central
od 2000-01-01 do Před 1 rokem
Open Access Digital Library
od 1997-01-01
Medline Complete (EBSCOhost)
od 1997-01-01 do 2015-11-30
Health & Medicine (ProQuest)
od 2000-01-01 do Před 1 rokem
PubMed
21441962
DOI
10.1038/bmt.2011.68
Knihovny.cz E-zdroje
- MeSH
- adenylátcyklasy genetika imunologie MeSH
- aktivace lymfocytů MeSH
- CD8-pozitivní T-lymfocyty imunologie MeSH
- Cytomegalovirus imunologie MeSH
- epitopy T-lymfocytární imunologie MeSH
- fosfoproteiny imunologie MeSH
- lidé MeSH
- peptidové fragmenty genetika imunologie MeSH
- proteiny virové matrix imunologie MeSH
- sekvence aminokyselin MeSH
- vakcíny proti cytomegalovirové infekci genetika imunologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Human CMV infects between 50-85% of healthy individuals and can cause live-threatening infections in immunocompromised patients. Therefore, peptide vaccination is being developed as a promising immunotherapeutic approach for treatment of patients at risk of CMV disease. The enzymatically inactive toxoid of Bordetella adenylate cyclase (CyaA-AC(-)) was shown to be an efficient tool for delivery of peptide epitopes and stimulation of Ag-specific T-cell immune responses. We investigated here the capacity of two CyaA-AC(-) constructs to deliver epitopes derived from the CMV phosphoprotein pp65 for activation of human T cells in vitro. Expansion of γ-IFN-secreting CMV-specific CD8(+) T cells, as well as increase of total IFN-γ and TNF-α production by PBMCs from CMV-seropositive donors were observed after in vitro stimulation with CyaA-AC(-) constructs carrying CMV epitopes, whereas limited activation of immune response occurred with free peptides. The activation of immune response was confirmed by expansion of CMV-specific T-cell clones and anti-CMV cytotoxic effect of stimulated PBMCs. These data open the way to clinical evaluation of CyaA-AC(-) constructs as tools for detection and expansion of CMV-specific T-cell immune responses for diagnostic and immunotherapeutic applications against CMV-associated diseases.
Cell and Molecular Microbiology Division Institute of Microbiology ASCR v v i Prague Czech Republic
Transfusion Department and Blood Bank of Brno Faculty Hospital Brno Czech Republic
Citace poskytuje Crossref.org
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