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Impact of normalization method on experimental outcome using RT-qPCR in Staphylococcus aureus
L. Valihrach, K. Demnerova,
Journal of microbiological methods.
2012 ;
90 (3) :
214-6.
Language English Country Netherlands
Document type Journal Article, Research Support, Non-U.S. Gov't
Persistent link
https://www.medvik.cz/link/bmc13000797
- MeSH
- Genes, Bacterial MeSH
- RNA, Bacterial genetics MeSH
- DNA Gyrase genetics MeSH
- Gene Expression MeSH
- Real-Time Polymerase Chain Reaction standards MeSH
- Reverse Transcriptase Polymerase Chain Reaction standards MeSH
- Reference Standards MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Staphylococcus aureus genetics MeSH
- Transcriptome MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
We performed a gene expression study using RT-qPCR in Staphylococcus aureus. The influence of normalization method was investigated. We confirmed that a recent standard, using more reference genes, was the best normalization strategy. The application of the most commonly used reference genes in 2011 (gyrB and 16S rRNA gene) failed.
References provided by Crossref.org
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