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Detection of NDM-1, VIM-1, KPC, OXA-48, and OXA-162 carbapenemases by matrix-assisted laser desorption ionization-time of flight mass spectrometry
J. Hrabák, V. Studentová, R. Walková, H. Zemlicková, V. Jakubu, E. Chudácková, M. Gniadkowski, Y. Pfeifer, JD. Perry, K. Wilkinson, T. Bergerová,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't, Validation Study
NLK
Free Medical Journals
from 1975 to 6 months ago
Freely Accessible Science Journals
from 1995 to 6 months ago
PubMed Central
from 1975 to 1 year ago
Europe PubMed Central
from 1975 to 6 months ago
Open Access Digital Library
from 1975-01-01
Open Access Digital Library
from 1975-01-01
PubMed
22553235
DOI
10.1128/jcm.01002-12
Knihovny.cz E-resources
- MeSH
- Acinetobacter baumannii enzymology MeSH
- Bacterial Proteins metabolism MeSH
- beta-Lactamases metabolism MeSH
- Enterobacteriaceae enzymology MeSH
- Enterobacteriaceae Infections microbiology MeSH
- Hydrolysis MeSH
- Humans MeSH
- Microbial Sensitivity Tests methods MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods MeSH
- Thienamycins metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Validation Study MeSH
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a potentially useful tool for the detection of antimicrobial resistance, especially that conferred by β-lactamases. Here we describe a modification of a previously reported MALDI-TOF MS meropenem hydrolysis assay. The modified method was validated on 108 carbapenemase-producing members of the Enterobacteriaceae, two NDM-1-producing Acinetobacter baumannii isolates, and 35 carbapenem-resistant enterobacteria producing no carbapenemase. The detection of carbapenemases by MALDI-TOF MS seems to be a powerful, quick, and cost-effective method for microbiological laboratories.
References provided by Crossref.org
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