-
Je něco špatně v tomto záznamu ?
New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations
D. Horák, J. Kučerová, L. Korecká, B. Jankovičová, J. Palarčík, P. Mikulášek, Z. Bílková,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Medline Complete (EBSCOhost)
od 2012-06-01 do Před 1 rokem
Wiley Online Library (archiv)
od 2001-01-01 do 2012-12-31
PubMed
22411761
DOI
10.1002/mabi.201100393
Knihovny.cz E-zdroje
- MeSH
- biokatalýza MeSH
- chromatografie afinitní metody MeSH
- imobilizované proteiny chemie MeSH
- imunoglobulin G chemie MeSH
- kyseliny polymethakrylové chemická syntéza MeSH
- lidé MeSH
- magnety MeSH
- mikrosféry MeSH
- mikroskopie elektronová rastrovací MeSH
- polyhydroxyethylmethakrylát chemická syntéza MeSH
- polymerizace MeSH
- spektrofotometrie infračervená MeSH
- trypsin chemie MeSH
- železité sloučeniny chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Magnetic macroporous PGMA and PHEMA microspheres containing carboxyl groups are synthesized by multi-step swelling and polymerization followed by precipitation of iron oxide inside the pores. The microspheres are characterized by SEM, IR spectroscopy, AAS, and zeta-potential measurements. Their functional groups enable bioactive ligands of various sizes and chemical structures to couple covalently. The applicability of these monodisperse magnetic microspheres in biospecific catalysis and bioaffinity separation is confirmed by coupling with the enzyme trypsin and huIgG. Trypsin-modified magnetic PGMA-COOH and PHEMA-COOH microspheres are investigated in terms of their enzyme activity, operational and storage stability. The presence of IgG molecules on microspheres is confirmed.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc13000917
- 003
- CZ-PrNML
- 005
- 20130110113956.0
- 007
- ta
- 008
- 130108s2012 gw f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1002/mabi.201100393 $2 doi
- 035 __
- $a (PubMed)22411761
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Horák, Daniel $u Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic. horak@imc.cas.cz
- 245 10
- $a New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations / $c D. Horák, J. Kučerová, L. Korecká, B. Jankovičová, J. Palarčík, P. Mikulášek, Z. Bílková,
- 520 9_
- $a Magnetic macroporous PGMA and PHEMA microspheres containing carboxyl groups are synthesized by multi-step swelling and polymerization followed by precipitation of iron oxide inside the pores. The microspheres are characterized by SEM, IR spectroscopy, AAS, and zeta-potential measurements. Their functional groups enable bioactive ligands of various sizes and chemical structures to couple covalently. The applicability of these monodisperse magnetic microspheres in biospecific catalysis and bioaffinity separation is confirmed by coupling with the enzyme trypsin and huIgG. Trypsin-modified magnetic PGMA-COOH and PHEMA-COOH microspheres are investigated in terms of their enzyme activity, operational and storage stability. The presence of IgG molecules on microspheres is confirmed.
- 650 _2
- $a biokatalýza $7 D055162
- 650 _2
- $a chromatografie afinitní $x metody $7 D002846
- 650 _2
- $a železité sloučeniny $x chemie $7 D005290
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a imobilizované proteiny $x chemie $7 D055767
- 650 _2
- $a imunoglobulin G $x chemie $7 D007074
- 650 _2
- $a magnety $7 D059346
- 650 _2
- $a mikroskopie elektronová rastrovací $7 D008855
- 650 _2
- $a mikrosféry $7 D008863
- 650 _2
- $a polyhydroxyethylmethakrylát $x chemická syntéza $7 D011102
- 650 _2
- $a polymerizace $7 D058105
- 650 _2
- $a kyseliny polymethakrylové $x chemická syntéza $7 D011109
- 650 _2
- $a spektrofotometrie infračervená $7 D013055
- 650 _2
- $a trypsin $x chemie $7 D014357
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Kučerová, Jana
- 700 1_
- $a Korecká, Lucie
- 700 1_
- $a Jankovičová, Barbora
- 700 1_
- $a Palarčík, Jiří
- 700 1_
- $a Mikulášek, Petr
- 700 1_
- $a Bílková, Zuzana
- 773 0_
- $w MED00006593 $t Macromolecular bioscience $x 1616-5195 $g Roč. 12, č. 5 (2012), s. 647-55
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/22411761 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20130108 $b ABA008
- 991 __
- $a 20130110114102 $b ABA008
- 999 __
- $a ok $b bmc $g 963699 $s 799081
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2012 $b 12 $c 5 $d 647-55 $i 1616-5195 $m Macromolecular bioscience $n Macromol Biosci $x MED00006593
- LZP __
- $a Pubmed-20130108
