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Male-specific repeats in wild Bovidae
K. Cabelova, S. Kubickova, H. Cernohorska, J. Rubes,
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Y Chromosome chemistry genetics MeSH
- Species Specificity MeSH
- Fluorescent Dyes chemistry MeSH
- Phylogeny MeSH
- In Situ Hybridization, Fluorescence veterinary MeSH
- Laser Capture Microdissection methods veterinary MeSH
- Metaphase MeSH
- Evolution, Molecular MeSH
- Genetics, Population methods MeSH
- Repetitive Sequences, Nucleic Acid * MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA methods veterinary MeSH
- Sequence Alignment MeSH
- Sex Factors MeSH
- Cattle classification genetics MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Cattle classification genetics MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
In this study, we investigated repetitive sequences localized on Y chromosomes. Repetitive DNA sequences represent a substantial part of the eukaryotic genome and, among them, a large portion comprises sequences repeated in tandem. Efficient and rapid isolation of repeat units is possible due to a laser microdissection technique used for Y chromosome separation, followed by polymerase chain reaction (PCR), cloning, and sequence analysis. We applied the derived repeat units to members of nine tribes within the Bovidae. Apart from the Y chromosomes of Bos taurus and Bubalus bubalis, where we used known sequences of repetition, the derived sequences were used as probes for fluorescent in situ cross-hybridization to members of the nine tribes of the Bovidae. We investigated the distribution of repeat units within the tribes and their localization on the Y chromosome. Sharing of sequence variants would indicate common descent, while the rapid horizontal evolution should allow discrimination between closely related species or subspecies.
References provided by Crossref.org
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- $a In this study, we investigated repetitive sequences localized on Y chromosomes. Repetitive DNA sequences represent a substantial part of the eukaryotic genome and, among them, a large portion comprises sequences repeated in tandem. Efficient and rapid isolation of repeat units is possible due to a laser microdissection technique used for Y chromosome separation, followed by polymerase chain reaction (PCR), cloning, and sequence analysis. We applied the derived repeat units to members of nine tribes within the Bovidae. Apart from the Y chromosomes of Bos taurus and Bubalus bubalis, where we used known sequences of repetition, the derived sequences were used as probes for fluorescent in situ cross-hybridization to members of the nine tribes of the Bovidae. We investigated the distribution of repeat units within the tribes and their localization on the Y chromosome. Sharing of sequence variants would indicate common descent, while the rapid horizontal evolution should allow discrimination between closely related species or subspecies.
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