-
Something wrong with this record ?
The use of hydrophilic poly(N,N-dimethylacrylamide) for promoting engulfment of magnetic gamma-Fe2O3 nanoparticles by mammalian cells
BA. Zasońska, N. Boiko, D. Horák, O. Klyuchivska, H. Macková, MJ. Benes, M. Babic, M. Trchová, J. Hromádková, R. Stoika,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23621005
DOI
10.1166/jbn.2013.1552
Knihovny.cz E-resources
- MeSH
- Acrylamides chemistry pharmacology MeSH
- Cell Line MeSH
- Microscopy, Fluorescence MeSH
- Hydrophobic and Hydrophilic Interactions drug effects MeSH
- Magnetic Phenomena MeSH
- Magnets * MeSH
- Macrophages cytology drug effects metabolism MeSH
- Mice MeSH
- Nanoparticles chemistry ultrastructure MeSH
- Polymerization drug effects MeSH
- Scattering, Radiation MeSH
- Mammals metabolism MeSH
- Spectroscopy, Fourier Transform Infrared MeSH
- Light MeSH
- Particle Size MeSH
- Ferric Compounds pharmacology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
gamma-Fe2O3 nanoparticles obtained by coprecipitation of Fe(II) and Fe(III) chlorides with a base and subsequent oxidation were coated with a shell of hydrophilic biocompatible poly(N,N-dimethylacrylamide) (PDMAAm). Various initiators were attached to the iron oxide surface to enable the use of the "grafting-from" approach for immobilization of PDMAAm. They included 2,2'-azobis(2-methylpropanimidamide) dihydrochloride (AMPA), 2,2'-azobis(N-hydroxy-2-methylpropanimidamide) dihydrochloride (ABHA) and 4-cyano-4-{[1-cyano-3-(N-hydroxycarbamoyl)-1-methylpropyl]azo}pentanoic acid (CCHPA). Engulfment of PDMAAm-coated y-Fe2O3 nanoparticles by murine J774.2 macrophages was investigated. Only some nanoparticles were engulfed by the macrophages. PDMAAm-AMPA-gamma-Fe2O3 and PDMAAm-ABHA-y-Fe2O3 nanoparticles were rapidly engulfed by the cells. In contrast, neat y-Fe2O3 and PDMAAm-CCHPA-gamma-Fe2O3 particles induced formation of transparent vacuoles indicating toxicity of the particles. Thus, PDMAAm-coated AMPA- and ABHA-gamma-Fe2O3 nanoparticles can be recommended as non-toxic labels for mammalian cells.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc13023918
- 003
- CZ-PrNML
- 005
- 20130710095631.0
- 007
- ta
- 008
- 130703s2013 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1166/jbn.2013.1552 $2 doi
- 035 __
- $a (PubMed)23621005
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Zasońska, Beata Anna $u Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovského Sq. 2, 162 06 Prague 6, Czech Republic.
- 245 14
- $a The use of hydrophilic poly(N,N-dimethylacrylamide) for promoting engulfment of magnetic gamma-Fe2O3 nanoparticles by mammalian cells / $c BA. Zasońska, N. Boiko, D. Horák, O. Klyuchivska, H. Macková, MJ. Benes, M. Babic, M. Trchová, J. Hromádková, R. Stoika,
- 520 9_
- $a gamma-Fe2O3 nanoparticles obtained by coprecipitation of Fe(II) and Fe(III) chlorides with a base and subsequent oxidation were coated with a shell of hydrophilic biocompatible poly(N,N-dimethylacrylamide) (PDMAAm). Various initiators were attached to the iron oxide surface to enable the use of the "grafting-from" approach for immobilization of PDMAAm. They included 2,2'-azobis(2-methylpropanimidamide) dihydrochloride (AMPA), 2,2'-azobis(N-hydroxy-2-methylpropanimidamide) dihydrochloride (ABHA) and 4-cyano-4-{[1-cyano-3-(N-hydroxycarbamoyl)-1-methylpropyl]azo}pentanoic acid (CCHPA). Engulfment of PDMAAm-coated y-Fe2O3 nanoparticles by murine J774.2 macrophages was investigated. Only some nanoparticles were engulfed by the macrophages. PDMAAm-AMPA-gamma-Fe2O3 and PDMAAm-ABHA-y-Fe2O3 nanoparticles were rapidly engulfed by the cells. In contrast, neat y-Fe2O3 and PDMAAm-CCHPA-gamma-Fe2O3 particles induced formation of transparent vacuoles indicating toxicity of the particles. Thus, PDMAAm-coated AMPA- and ABHA-gamma-Fe2O3 nanoparticles can be recommended as non-toxic labels for mammalian cells.
- 650 _2
- $a akrylamidy $x chemie $x farmakologie $7 D000178
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a buněčné linie $7 D002460
- 650 _2
- $a železité sloučeniny $x farmakologie $7 D005290
- 650 _2
- $a hydrofobní a hydrofilní interakce $x účinky léků $7 D057927
- 650 _2
- $a světlo $7 D008027
- 650 _2
- $a makrofágy $x cytologie $x účinky léků $x metabolismus $7 D008264
- 650 _2
- $a magnetické jevy $7 D060328
- 650 12
- $a magnety $7 D059346
- 650 _2
- $a savci $x metabolismus $7 D008322
- 650 _2
- $a myši $7 D051379
- 650 _2
- $a fluorescenční mikroskopie $7 D008856
- 650 _2
- $a nanočástice $x chemie $x ultrastruktura $7 D053758
- 650 _2
- $a velikost částic $7 D010316
- 650 _2
- $a polymerizace $x účinky léků $7 D058105
- 650 _2
- $a radiační rozptyl $7 D012542
- 650 _2
- $a spektroskopie infračervená s Fourierovou transformací $7 D017550
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Boiko, Nataliya $u -
- 700 1_
- $a Horák, Daniel $u -
- 700 1_
- $a Klyuchivska, Olga $u -
- 700 1_
- $a Macková, Hana $u -
- 700 1_
- $a Benes, Milan J $u -
- 700 1_
- $a Babic, Michal $u -
- 700 1_
- $a Trchová, Miroslava $u -
- 700 1_
- $a Hromádková, Jirina $u -
- 700 1_
- $a Stoika, Rostyslav $u -
- 773 0_
- $w MED00169220 $t Journal of biomedical nanotechnology $x 1550-7033 $g Roč. 9, č. 3 (2013), s. 479-91
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/23621005 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20130703 $b ABA008
- 991 __
- $a 20130710100054 $b ABA008
- 999 __
- $a ok $b bmc $g 987598 $s 822298
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2013 $b 9 $c 3 $d 479-91 $i 1550-7033 $m Journal of biomedical nanotechnology $n J. biomed. nanotechnol. $x MED00169220
- LZP __
- $a Pubmed-20130703
