The use of hydrophilic poly(N,N-dimethylacrylamide) for promoting engulfment of magnetic gamma-Fe2O3 nanoparticles by mammalian cells
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23621005
DOI
10.1166/jbn.2013.1552
Knihovny.cz E-resources
- MeSH
- Acrylamides chemistry pharmacology MeSH
- Cell Line MeSH
- Microscopy, Fluorescence MeSH
- Hydrophobic and Hydrophilic Interactions drug effects MeSH
- Magnetic Phenomena MeSH
- Magnets * MeSH
- Macrophages cytology drug effects metabolism MeSH
- Mice MeSH
- Nanoparticles chemistry ultrastructure MeSH
- Polymerization drug effects MeSH
- Scattering, Radiation MeSH
- Mammals metabolism MeSH
- Spectroscopy, Fourier Transform Infrared MeSH
- Light MeSH
- Particle Size MeSH
- Ferric Compounds pharmacology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Acrylamides MeSH
- ferric oxide MeSH Browser
- poly(N,N-dimethylacrylamide) MeSH Browser
- Ferric Compounds MeSH
gamma-Fe2O3 nanoparticles obtained by coprecipitation of Fe(II) and Fe(III) chlorides with a base and subsequent oxidation were coated with a shell of hydrophilic biocompatible poly(N,N-dimethylacrylamide) (PDMAAm). Various initiators were attached to the iron oxide surface to enable the use of the "grafting-from" approach for immobilization of PDMAAm. They included 2,2'-azobis(2-methylpropanimidamide) dihydrochloride (AMPA), 2,2'-azobis(N-hydroxy-2-methylpropanimidamide) dihydrochloride (ABHA) and 4-cyano-4-{[1-cyano-3-(N-hydroxycarbamoyl)-1-methylpropyl]azo}pentanoic acid (CCHPA). Engulfment of PDMAAm-coated y-Fe2O3 nanoparticles by murine J774.2 macrophages was investigated. Only some nanoparticles were engulfed by the macrophages. PDMAAm-AMPA-gamma-Fe2O3 and PDMAAm-ABHA-y-Fe2O3 nanoparticles were rapidly engulfed by the cells. In contrast, neat y-Fe2O3 and PDMAAm-CCHPA-gamma-Fe2O3 particles induced formation of transparent vacuoles indicating toxicity of the particles. Thus, PDMAAm-coated AMPA- and ABHA-gamma-Fe2O3 nanoparticles can be recommended as non-toxic labels for mammalian cells.
References provided by Crossref.org
