Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability

M. Kunova, K. Matulka, L. Eiselleova, A. Salykin, I. Kubikova, S. Kyrylenko, A. Hampl, P. Dvorak,

. 2013 ; 2 (4) : 246-54.

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc13023938

The generation of human pluripotent stem cells (hPSCs) of sufficient quantity and quality remains a major challenge for biomedical application. Here we present an efficient feeder-free, high-density monolayer system in which hPSCs become SSEA-3-high and gradually more viable than their feeder-dependent counterparts without changes attributed to culture adaptation. As a consequence, monolayer hPSCs possess advantages over their counterparts in embryoid body development, teratoma formation, freezing as a single-cell suspension, and colony-forming efficiency. Importantly, this monolayer culture system is reversible, preserving the competence of hPSCs to gradually reacquire features of colony growth, if necessary. Therefore, the monolayer culture system is highly suitable for long-term, large-scale propagation of hPSCs, which is necessary in drug development and pluripotent stem cell-based therapies.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc13023938
003      
CZ-PrNML
005      
20130723095351.0
007      
ta
008      
130703s2013 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.5966/sctm.2012-0081 $2 doi
035    __
$a (PubMed)23486835
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Kunova, Michaela $u Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
245    10
$a Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability / $c M. Kunova, K. Matulka, L. Eiselleova, A. Salykin, I. Kubikova, S. Kyrylenko, A. Hampl, P. Dvorak,
520    9_
$a The generation of human pluripotent stem cells (hPSCs) of sufficient quantity and quality remains a major challenge for biomedical application. Here we present an efficient feeder-free, high-density monolayer system in which hPSCs become SSEA-3-high and gradually more viable than their feeder-dependent counterparts without changes attributed to culture adaptation. As a consequence, monolayer hPSCs possess advantages over their counterparts in embryoid body development, teratoma formation, freezing as a single-cell suspension, and colony-forming efficiency. Importantly, this monolayer culture system is reversible, preserving the competence of hPSCs to gradually reacquire features of colony growth, if necessary. Therefore, the monolayer culture system is highly suitable for long-term, large-scale propagation of hPSCs, which is necessary in drug development and pluripotent stem cell-based therapies.
650    _2
$a zvířata $7 D000818
650    _2
$a biologické markery $x metabolismus $7 D015415
650    _2
$a buněčné kultury $x metody $7 D018929
650    _2
$a buněčné linie $7 D002460
650    _2
$a proliferace buněk $7 D049109
650    _2
$a viabilita buněk $7 D002470
650    _2
$a analýza kolonii tvořících jednotek $7 D003114
650    _2
$a podkladové buňky $x cytologie $7 D061252
650    _2
$a lidé $7 D006801
650    _2
$a indukované pluripotentní kmenové buňky $x cytologie $x metabolismus $7 D057026
650    _2
$a myši $7 D051379
650    _2
$a pluripotentní kmenové buňky $x cytologie $x metabolismus $7 D039904
650    _2
$a teratom $x patologie $7 D013724
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Matulka, Kamil $u -
700    1_
$a Eiselleova, Livia $u -
700    1_
$a Salykin, Anton $u -
700    1_
$a Kubikova, Iva $u -
700    1_
$a Kyrylenko, Sergiy $u -
700    1_
$a Hampl, Ales $u -
700    1_
$a Dvorak, Petr $u -
773    0_
$w MED00181744 $t Stem cells translational medicine $x 2157-6564 $g Roč. 2, č. 4 (2013), s. 246-54
856    41
$u https://pubmed.ncbi.nlm.nih.gov/23486835 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20130703 $b ABA008
991    __
$a 20130723095839 $b ABA008
999    __
$a ok $b bmc $g 987618 $s 822318
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2013 $b 2 $c 4 $d 246-54 $i 2157-6564 $m Stem cells translational medicine $n Stem Cells Transl Med $x MED00181744
LZP    __
$a Pubmed-20130703

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace