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Efficient iron uptake via a reductive mechanism in procyclic Trypanosoma brucei
J. Mach, J. Tachezy, R. Sutak,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
ProQuest Central
from 2000-12-01 to 2023-12-31
Medline Complete (EBSCOhost)
from 2007-06-01 to 6 months ago
Health & Medicine (ProQuest)
from 2000-12-01 to 2023-12-31
Public Health Database (ProQuest)
from 2000-12-01 to 2023-12-31
PubMed
22924933
DOI
10.1645/ge-3237.1
Knihovny.cz E-resources
- MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- FMN Reductase metabolism MeSH
- Host-Parasite Interactions MeSH
- Mitochondria metabolism MeSH
- Oxidation-Reduction MeSH
- Trypanosoma brucei brucei metabolism MeSH
- Ferric Compounds metabolism MeSH
- Ferrous Compounds metabolism MeSH
- Iron metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The bloodstream form of Trypanosoma brucei acquires iron from transferrin by receptor-mediated endocytosis. However, it is unknown how procyclic forms that cannot bind transferrin acquire iron. Here, we show that the procyclic form of T. brucei efficiently takes up iron from ferric complexes via a reductive mechanism and that iron obtained using this mechanism is transported to, and used in, the mitochondria. The affinity of the transport system is comparable to that of Saccharomyces cerevisiae , with an apparent K(m) of 0.85 μM.
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- $a The bloodstream form of Trypanosoma brucei acquires iron from transferrin by receptor-mediated endocytosis. However, it is unknown how procyclic forms that cannot bind transferrin acquire iron. Here, we show that the procyclic form of T. brucei efficiently takes up iron from ferric complexes via a reductive mechanism and that iron obtained using this mechanism is transported to, and used in, the mitochondria. The affinity of the transport system is comparable to that of Saccharomyces cerevisiae , with an apparent K(m) of 0.85 μM.
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