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A quantitative objective method for the evaluation of anti-sperm cell-mediated immunity in humans
DG Dimitrov, R Sedlak, K Nouza, R Kinsky
Jazyk angličtina Země Nizozemsko
Grantová podpora
PL180
MZ0
CEP - Centrální evidence projektů
- MeSH
- autoimunita MeSH
- buněčná imunita * imunologie MeSH
- inhibice migrace buněk MeSH
- lidé MeSH
- mužská infertilita diagnóza imunologie MeSH
- spermie * imunologie MeSH
- ženská infertilita diagnóza imunologie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
Anti-sperm cell-mediated immunity (CMI) is considered as a crucial facet of infertility in patients of both sexes. A precise and objective method is designed, based on a one-step agarose leukocyte migration inhibition factor (LMIF) assay. The migration areas are evaluated by a computer-assisted image analysis system. Optimal concentrations of leukocytes and sperm, as well as technical conditions are described. The radial migration indexes (RMI) and area migration indexes (AMI) are computed and expressed as a migration index (MI) percentage for each patient or control. Preliminary clinical results indicate a highly significant association between migration inhibition and cases of 'immunopathological infertility'. The method described is considered a promising tool for a rapid and quantitative evaluation of a suspected anti-sperm CMI in infertile and recurrently aborting patients.
Citace poskytuje Crossref.org
Literatura
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- $a Anti-sperm cell-mediated immunity (CMI) is considered as a crucial facet of infertility in patients of both sexes. A precise and objective method is designed, based on a one-step agarose leukocyte migration inhibition factor (LMIF) assay. The migration areas are evaluated by a computer-assisted image analysis system. Optimal concentrations of leukocytes and sperm, as well as technical conditions are described. The radial migration indexes (RMI) and area migration indexes (AMI) are computed and expressed as a migration index (MI) percentage for each patient or control. Preliminary clinical results indicate a highly significant association between migration inhibition and cases of 'immunopathological infertility'. The method described is considered a promising tool for a rapid and quantitative evaluation of a suspected anti-sperm CMI in infertile and recurrently aborting patients.
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