-
Je něco špatně v tomto záznamu ?
A transcriptome-wide RNAi screen in the Drosophila ovary reveals factors of the germline piRNA pathway
B. Czech, JB. Preall, J. McGinn, GJ. Hannon,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem
NLK
Cell Press Free Archives
od 1997-12-01 do Před 1 rokem
Free Medical Journals
od 1997 do Před 1 rokem
Free Medical Journals
od 1997 do Před 1 rokem
Open Access Digital Library
od 1997-12-01
Elsevier Open Access Journals
od 1997-12-01 do 2023-06-15
Elsevier Open Archive Journals
od 1997-12-01 do Před 1 rokem
- MeSH
- Argonaut proteiny genetika metabolismus MeSH
- Drosophila melanogaster genetika MeSH
- iniciační faktory genetika metabolismus MeSH
- malá interferující RNA genetika metabolismus MeSH
- ovarium fyziologie MeSH
- proteiny asociované s mikrotubuly genetika metabolismus MeSH
- proteiny Drosophily genetika metabolismus MeSH
- reprodukovatelnost výsledků MeSH
- RNA interference * MeSH
- stanovení celkové genové exprese metody MeSH
- transpozibilní elementy DNA * MeSH
- umlčování genů MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
The Drosophila piRNA pathway provides an RNA-based immune system that defends the germline genome against selfish genetic elements. Two interrelated branches of the piRNA system exist: somatic cells that support oogenesis only employ Piwi, whereas germ cells utilize a more elaborate pathway centered on the three gonad-specific Argonaute proteins (Piwi, Aubergine, and Argonaute 3). While several key factors of each branch have been identified, our current knowledge is insufficient to explain the complex workings of the piRNA machinery. Here, we report a reverse genetic screen spanning the ovarian transcriptome in an attempt to uncover the full repertoire of genes required for piRNA-mediated transposon silencing in the female germline. Our screen reveals key factors of piRNA-mediated transposon silencing, including the piRNA biogenesis factors CG2183 (GASZ) and Deadlock. Our data uncover a previously unanticipated set of factors preferentially required for repression of different transposon types.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc13031469
- 003
- CZ-PrNML
- 005
- 20131008104633.0
- 007
- ta
- 008
- 131002s2013 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.molcel.2013.04.007 $2 doi
- 035 __
- $a (PubMed)23665227
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Czech, Benjamin $u Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA. czech@cshl.edu
- 245 12
- $a A transcriptome-wide RNAi screen in the Drosophila ovary reveals factors of the germline piRNA pathway / $c B. Czech, JB. Preall, J. McGinn, GJ. Hannon,
- 520 9_
- $a The Drosophila piRNA pathway provides an RNA-based immune system that defends the germline genome against selfish genetic elements. Two interrelated branches of the piRNA system exist: somatic cells that support oogenesis only employ Piwi, whereas germ cells utilize a more elaborate pathway centered on the three gonad-specific Argonaute proteins (Piwi, Aubergine, and Argonaute 3). While several key factors of each branch have been identified, our current knowledge is insufficient to explain the complex workings of the piRNA machinery. Here, we report a reverse genetic screen spanning the ovarian transcriptome in an attempt to uncover the full repertoire of genes required for piRNA-mediated transposon silencing in the female germline. Our screen reveals key factors of piRNA-mediated transposon silencing, including the piRNA biogenesis factors CG2183 (GASZ) and Deadlock. Our data uncover a previously unanticipated set of factors preferentially required for repression of different transposon types.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a Argonaut proteiny $x genetika $x metabolismus $7 D060565
- 650 12
- $a transpozibilní elementy DNA $7 D004251
- 650 _2
- $a proteiny Drosophily $x genetika $x metabolismus $7 D029721
- 650 _2
- $a Drosophila melanogaster $x genetika $7 D004331
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a stanovení celkové genové exprese $x metody $7 D020869
- 650 _2
- $a umlčování genů $7 D020868
- 650 _2
- $a proteiny asociované s mikrotubuly $x genetika $x metabolismus $7 D008869
- 650 _2
- $a ovarium $x fyziologie $7 D010053
- 650 _2
- $a iniciační faktory $x genetika $x metabolismus $7 D010448
- 650 12
- $a RNA interference $7 D034622
- 650 _2
- $a malá interferující RNA $x genetika $x metabolismus $7 D034741
- 650 _2
- $a reprodukovatelnost výsledků $7 D015203
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a Research Support, N.I.H., Extramural $7 D052061
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Preall, Jonathan B $u -
- 700 1_
- $a McGinn, Jon $u -
- 700 1_
- $a Hannon, Gregory J $u -
- 773 0_
- $w MED00011398 $t Molecular cell $x 1097-4164 $g Roč. 50, č. 5 (2013), s. 749-61
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/23665227 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20131002 $b ABA008
- 991 __
- $a 20131008105155 $b ABA008
- 999 __
- $a ok $b bmc $g 995556 $s 829914
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2013 $b 50 $c 5 $d 749-61 $i 1097-4164 $m Molecular cell $n Mol Cell $x MED00011398
- LZP __
- $a Pubmed-20131002
