Differences in the level of cold acclimation and cryoprotection estimated as ice nucleation activity in snow algae (Chlamydomonas cf. nivalis and Chloromonas nivalis), lichen symbiotic algae (Trebouxia asymmetrica, Trebouxia erici and Trebouxia glomerata), and a mesophilic strain (Chlamydomonas reinhardti) were evaluated. Ice nucleation activity was measured using the freezing droplet method. Measurements were performed using suspensions of cells of A750 (absorbance at 750 nm) ~ 1, 0.1, 0.01 and 0.001 dilutions for each strain. The algae had lower ice nucleation activity, with the exception of Chloromonas nivalis contaminated by bacteria. The supercooling points of the snow algae were higher than those of lichen photobionts. The supercooling points of both, mesophilic and snow Chlamydomonas strains were similar. The lower freezing temperatures of the lichen algae may reflect either the more extreme and more variable environmental conditions of the original localities or the different cellular structure of the strains examined.

Institute of Botany Academy of Sciences of the Czech Republic Dukelska Trebon Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc13031481

003      

CZ-PrNML

005      

20131004100522.0

007      

ta

008      

131002s2013 enk f 000 0|eng||

009      

AR

035    __

$a (PubMed)23625082

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a enk

100    1_

$a Kviderova, Jana $u Institute of Botany, Academy of Sciences of the Czech Republic, Dukelska, Trebon, Czech Republic. kviderova@butbn.cas.cz

245    14

$a The ice nucleation activity of extremophilic algae / $c J. Kviderova, J. Hajek, RM. Worland,

520    9_

$a Differences in the level of cold acclimation and cryoprotection estimated as ice nucleation activity in snow algae (Chlamydomonas cf. nivalis and Chloromonas nivalis), lichen symbiotic algae (Trebouxia asymmetrica, Trebouxia erici and Trebouxia glomerata), and a mesophilic strain (Chlamydomonas reinhardti) were evaluated. Ice nucleation activity was measured using the freezing droplet method. Measurements were performed using suspensions of cells of A750 (absorbance at 750 nm) ~ 1, 0.1, 0.01 and 0.001 dilutions for each strain. The algae had lower ice nucleation activity, with the exception of Chloromonas nivalis contaminated by bacteria. The supercooling points of the snow algae were higher than those of lichen photobionts. The supercooling points of both, mesophilic and snow Chlamydomonas strains were similar. The lower freezing temperatures of the lichen algae may reflect either the more extreme and more variable environmental conditions of the original localities or the different cellular structure of the strains examined.

650    _2

$a aklimatizace $7 D000064

650    _2

$a Chlamydomonas $x chemie $x cytologie $x fyziologie $7 D002696

650    _2

$a Chlorophyta $x chemie $x cytologie $x fyziologie $7 D000460

650    _2

$a krystalizace $7 D003460

650    _2

$a zmrazování $7 D005615

650    _2

$a led $x analýza $7 D007053

650    _2

$a lišejníky $x fyziologie $7 D008011

650    _2

$a symbióza $7 D013559

655    _2

$a časopisecké články $7 D016428

655    _2

$a práce podpořená grantem $7 D013485

700    1_

$a Hajek, Josef $u -

700    1_

$a Worland, Roger M $u -

773    0_

$w MED00008393 $t Cryo letters $x 0143-2044 $g Roč. 34, č. 2 (2013), s. 137-48

856    41

$u https://pubmed.ncbi.nlm.nih.gov/23625082 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20131002 $b ABA008

991    __

$a 20131004101040 $b ABA008

999    __

$a ok $b bmc $g 995568 $s 829926

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2013 $b 34 $c 2 $d 137-48 $i 0143-2044 $m Cryo-letters $n Cryo Letters $x MED00008393

LZP    __

$a Pubmed-20131002