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Estimation of the quantity of bacteria encapsulated in Lentikats Biocatalyst via phospholipid fatty acids content: a preliminary study

J. Trögl, I. Jirková, P. Zemánková, V. Pilařová, P. Dáňová, J. Pavlorková, P. Kuráň, J. Popelka, L. Křiklavová

. 2013 ; 58 (2) : 135-140.

Language English Country Czech Republic

Document type Journal Article, Research Support, Non-U.S. Gov't

The content of phospholipid fatty acids (PLFA) was determined in samples of polyvinyl alcohol lenses (Lentikats Biocatalyst, LB) with encapsulated Paracoccus denitrificans withdrawn during long-term denitrification experiments. The total PLFA content correlated highly with specific denitrification activities of LB as well as biomass estimation based on image analyses of microscopic photos. The results confirmed the applicability of PLFA determination for estimation of the amount of living encapsulated microbial biomass during biotechnological applications.

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