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Impedance spectroscopy of bilayer lipid membranes self-assembled on agar support - interaction with HDL
M. Legin, G. Laputková, J. Sabo, L. Vojcíková
Language English Country Czech Republic
Document type Journal Article, Research Support, Non-U.S. Gov't
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- MeSH
- Agar chemistry MeSH
- Models, Chemical MeSH
- Electric Impedance MeSH
- Electric Capacitance MeSH
- Phosphatidylcholines chemistry MeSH
- Lipid Bilayers * MeSH
- Lipoproteins, HDL chemistry MeSH
- Spectrum Analysis methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The electrical properties of the supported lipid bilayer membrane (s-BLM) of egg phosphatidylcholine (PC) self-assembled on agar surface were examined. To characterize the insulating properties of s-BLMs, electrochemical impedance spectroscopy (EIS) was used. The analysis of impedance spectra in terms of an equivalent circuit of agar/electrolyte and agar/s-BLM/electrolyte in the frequency range of 0.1 Hz-10 kHz was performed. The high-density lipoproteins (HDL)/s-BLM interaction in the concentration range from 20 microg/ml to 80 microg/ml of HDL was investigated. It is evident that treatment of s-BLM with HDL resulted in an increase of the lipid film resistance and a decrease of membrane capacitance.
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- $a Legiň, Michal $u Department of Medical Biophysics, Faculty of Medicine, Pavol Jozef Safárik University, Kosice, Slovakia. legin@lf.upjs.sk $7 _AN049633
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- $a The electrical properties of the supported lipid bilayer membrane (s-BLM) of egg phosphatidylcholine (PC) self-assembled on agar surface were examined. To characterize the insulating properties of s-BLMs, electrochemical impedance spectroscopy (EIS) was used. The analysis of impedance spectra in terms of an equivalent circuit of agar/electrolyte and agar/s-BLM/electrolyte in the frequency range of 0.1 Hz-10 kHz was performed. The high-density lipoproteins (HDL)/s-BLM interaction in the concentration range from 20 microg/ml to 80 microg/ml of HDL was investigated. It is evident that treatment of s-BLM with HDL resulted in an increase of the lipid film resistance and a decrease of membrane capacitance.
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