Impedance spectroscopy of bilayer lipid membranes self-assembled on agar support - interaction with HDL
Jazyk angličtina Země Česko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
17552891
DOI
10.33549/physiolres.931305
PII: 1305
Knihovny.cz E-zdroje
- MeSH
- agar chemie MeSH
- chemické modely MeSH
- elektrická impedance MeSH
- elektrická kapacitance MeSH
- fosfatidylcholiny chemie MeSH
- lipidové dvojvrstvy * MeSH
- lipoproteiny HDL chemie MeSH
- spektrální analýza metody MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- agar MeSH
- fosfatidylcholiny MeSH
- lipidové dvojvrstvy * MeSH
- lipoproteiny HDL MeSH
The electrical properties of the supported lipid bilayer membrane (s-BLM) of egg phosphatidylcholine (PC) self-assembled on agar surface were examined. To characterize the insulating properties of s-BLMs, electrochemical impedance spectroscopy (EIS) was used. The analysis of impedance spectra in terms of an equivalent circuit of agar/electrolyte and agar/s-BLM/electrolyte in the frequency range of 0.1 Hz-10 kHz was performed. The high-density lipoproteins (HDL)/s-BLM interaction in the concentration range from 20 microg/ml to 80 microg/ml of HDL was investigated. It is evident that treatment of s-BLM with HDL resulted in an increase of the lipid film resistance and a decrease of membrane capacitance.
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