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The pharmacological activation of adenosine A1 and A 3 receptors does not modulate the long- or short-term repopulating ability of hematopoietic stem and multipotent progenitor cells in mice
M. Hofer, M. Pospíšil, Z. Hoferová, D. Komůrková, P. Páral, F. Savvulidi, L. Sefc,
Language English Country Netherlands
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
PubMed Central
from 2004 to 6 months ago
Europe PubMed Central
from 2004 to 6 months ago
- MeSH
- Purinergic P1 Receptor Agonists pharmacology MeSH
- Hematopoietic Stem Cells drug effects physiology MeSH
- Hematopoiesis drug effects physiology MeSH
- Multipotent Stem Cells drug effects physiology MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Flow Cytometry MeSH
- Receptor, Adenosine A1 metabolism MeSH
- Receptor, Adenosine A3 metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
This study continues our earlier findings on the hematopoiesis-modulating effects of adenosine A1 and A3 receptor agonists that were performed on committed hematopoietic progenitor and precursor cell populations. In the earlier experiments, N (6)-cyclopentyladenosine (CPA), an adenosine A1 receptor agonist, was found to inhibit proliferation in the above-mentioned hematopoietic cell systems, whereas N (6)-(3-iodobenzyl)adenosine-5'-N-methyluronamide (IB-MECA), an adenosine A3 receptor agonist, was found to stimulate it. The topic of this study was to evaluate the possibility that the above-mentioned adenosine receptor agonists modulate the behavior of early hematopoietic progenitor cells and hematopoietic stem cells. Flow cytometric analysis of hematopoietic stem cells in mice was employed, as well as a functional test of hematopoietic stem and progenitor cells (HSPCs). These techniques enabled us to study the effect of the agonists on both short-term repopulating ability and long-term repopulating ability, representing multipotent progenitors and hematopoietic stem cells, respectively. In a series of studies, we did not find any significant effect of adenosine agonists on HSPCs in terms of their numbers, proliferation, or functional activity. Thus, it can be concluded that CPA and IB-MECA do not significantly influence the primitive hematopoietic stem and progenitor cell pool and that the hematopoiesis-modulating action of these adenosine receptor agonists is restricted to more mature compartments of hematopoietic progenitor and precursor cells.
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