Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

The pharmacological activation of adenosine A1 and A 3 receptors does not modulate the long- or short-term repopulating ability of hematopoietic stem and multipotent progenitor cells in mice

M. Hofer, M. Pospíšil, Z. Hoferová, D. Komůrková, P. Páral, F. Savvulidi, L. Sefc,

. 2013 ; 9 (2) : 207-14.

Language English Country Netherlands

Document type Journal Article, Research Support, Non-U.S. Gov't

This study continues our earlier findings on the hematopoiesis-modulating effects of adenosine A1 and A3 receptor agonists that were performed on committed hematopoietic progenitor and precursor cell populations. In the earlier experiments, N (6)-cyclopentyladenosine (CPA), an adenosine A1 receptor agonist, was found to inhibit proliferation in the above-mentioned hematopoietic cell systems, whereas N (6)-(3-iodobenzyl)adenosine-5'-N-methyluronamide (IB-MECA), an adenosine A3 receptor agonist, was found to stimulate it. The topic of this study was to evaluate the possibility that the above-mentioned adenosine receptor agonists modulate the behavior of early hematopoietic progenitor cells and hematopoietic stem cells. Flow cytometric analysis of hematopoietic stem cells in mice was employed, as well as a functional test of hematopoietic stem and progenitor cells (HSPCs). These techniques enabled us to study the effect of the agonists on both short-term repopulating ability and long-term repopulating ability, representing multipotent progenitors and hematopoietic stem cells, respectively. In a series of studies, we did not find any significant effect of adenosine agonists on HSPCs in terms of their numbers, proliferation, or functional activity. Thus, it can be concluded that CPA and IB-MECA do not significantly influence the primitive hematopoietic stem and progenitor cell pool and that the hematopoiesis-modulating action of these adenosine receptor agonists is restricted to more mature compartments of hematopoietic progenitor and precursor cells.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc14040969
003      
CZ-PrNML
005      
20140113110654.0
007      
ta
008      
140107s2013 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1007/s11302-012-9340-5 $2 doi
035    __
$a (PubMed)23242571
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Hofer, Michal
245    14
$a The pharmacological activation of adenosine A1 and A 3 receptors does not modulate the long- or short-term repopulating ability of hematopoietic stem and multipotent progenitor cells in mice / $c M. Hofer, M. Pospíšil, Z. Hoferová, D. Komůrková, P. Páral, F. Savvulidi, L. Sefc,
520    9_
$a This study continues our earlier findings on the hematopoiesis-modulating effects of adenosine A1 and A3 receptor agonists that were performed on committed hematopoietic progenitor and precursor cell populations. In the earlier experiments, N (6)-cyclopentyladenosine (CPA), an adenosine A1 receptor agonist, was found to inhibit proliferation in the above-mentioned hematopoietic cell systems, whereas N (6)-(3-iodobenzyl)adenosine-5'-N-methyluronamide (IB-MECA), an adenosine A3 receptor agonist, was found to stimulate it. The topic of this study was to evaluate the possibility that the above-mentioned adenosine receptor agonists modulate the behavior of early hematopoietic progenitor cells and hematopoietic stem cells. Flow cytometric analysis of hematopoietic stem cells in mice was employed, as well as a functional test of hematopoietic stem and progenitor cells (HSPCs). These techniques enabled us to study the effect of the agonists on both short-term repopulating ability and long-term repopulating ability, representing multipotent progenitors and hematopoietic stem cells, respectively. In a series of studies, we did not find any significant effect of adenosine agonists on HSPCs in terms of their numbers, proliferation, or functional activity. Thus, it can be concluded that CPA and IB-MECA do not significantly influence the primitive hematopoietic stem and progenitor cell pool and that the hematopoiesis-modulating action of these adenosine receptor agonists is restricted to more mature compartments of hematopoietic progenitor and precursor cells.
650    _2
$a zvířata $7 D000818
650    _2
$a průtoková cytometrie $7 D005434
650    _2
$a hematopoéza $x účinky léků $x fyziologie $7 D006410
650    _2
$a hematopoetické kmenové buňky $x účinky léků $x fyziologie $7 D006412
650    _2
$a myši $7 D051379
650    _2
$a myši inbrední C57BL $7 D008810
650    _2
$a multipotentní kmenové buňky $x účinky léků $x fyziologie $7 D039902
650    _2
$a agonisté purinergních receptorů P1 $x farmakologie $7 D058906
650    _2
$a receptor adenosinový A1 $x metabolismus $7 D043682
650    _2
$a receptor adenosinový A3 $x metabolismus $7 D043684
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Pospíšil, Milan $u -
700    1_
$a Hoferová, Zuzana $u -
700    1_
$a Komůrková, Denisa $u -
700    1_
$a Páral, Petr $u -
700    1_
$a Savvulidi, Filipp $u -
700    1_
$a Sefc, Luděk $u -
773    0_
$w MED00182192 $t Purinergic signalling $x 1573-9546 $g Roč. 9, č. 2 (2013), s. 207-14
856    41
$u https://pubmed.ncbi.nlm.nih.gov/23242571 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20140107 $b ABA008
991    __
$a 20140113111358 $b ABA008
999    __
$a ok $b bmc $g 1005365 $s 839481
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2013 $b 9 $c 2 $d 207-14 $i 1573-9546 $m Purinergic signalling $n Purinergic Signal $x MED00182192
LZP    __
$a Pubmed-20140107

Find record

Citation metrics

Loading data ...

Archiving options

Loading data ...