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Validation of RNA extraction procedures focused on micro RNA expression analysis

M. Remáková, M. Škoda, M. Faustová, J. Vencovský, P. Novota

. 2013 ; 59 (1) : 47-50.

Jazyk angličtina Země Česko

Typ dokumentu časopisecké články, práce podpořená grantem, validační studie

Perzistentní odkaz   https://www.medvik.cz/link/bmc14046115

Grantová podpora
NT12452 MZ0 CEP - Centrální evidence projektů

The sampling procedure is a crucial step in every kind of experiment. This is also true in gene expression profiling experiments, where high quality and sufficient quantity of extracted RNA plays a significant role in the experimental outcome. We have compared five different RNA extraction protocols from peripheral blood/PBMCs with the aim to define the most suitable method for the miRNA expression profiling experiments. Convincing results in terms of highest quantity and quality were obtained by the TRIzol-chloroform extraction method. The total RNA obtained using this method contained the highest portion of good-quality miRNA molecules, which was also confirmed by gene-specific real-time PCR experiments.

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