Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Validation of RNA extraction procedures focused on micro RNA expression analysis

M. Remáková, M. Škoda, M. Faustová, J. Vencovský, P. Novota

. 2013 ; 59 (1) : 47-50.

Language English Country Czech Republic

Document type Journal Article, Research Support, Non-U.S. Gov't, Validation Study

Persistent link   https://www.medvik.cz/link/bmc14046115

Grant support
NT12452 MZ0 CEP Register

The sampling procedure is a crucial step in every kind of experiment. This is also true in gene expression profiling experiments, where high quality and sufficient quantity of extracted RNA plays a significant role in the experimental outcome. We have compared five different RNA extraction protocols from peripheral blood/PBMCs with the aim to define the most suitable method for the miRNA expression profiling experiments. Convincing results in terms of highest quantity and quality were obtained by the TRIzol-chloroform extraction method. The total RNA obtained using this method contained the highest portion of good-quality miRNA molecules, which was also confirmed by gene-specific real-time PCR experiments.

000      
00000naa a2200000 a 4500
001      
bmc14046115
003      
CZ-PrNML
005      
20170623111942.0
007      
ta
008      
140212s2013 xr d f 000 0|eng||
009      
AR
035    __
$a (PubMed)23537528
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Mišunová, Martina $7 xx0165670 $u Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic
245    10
$a Validation of RNA extraction procedures focused on micro RNA expression analysis / $c M. Remáková, M. Škoda, M. Faustová, J. Vencovský, P. Novota
520    9_
$a The sampling procedure is a crucial step in every kind of experiment. This is also true in gene expression profiling experiments, where high quality and sufficient quantity of extracted RNA plays a significant role in the experimental outcome. We have compared five different RNA extraction protocols from peripheral blood/PBMCs with the aim to define the most suitable method for the miRNA expression profiling experiments. Convincing results in terms of highest quantity and quality were obtained by the TRIzol-chloroform extraction method. The total RNA obtained using this method contained the highest portion of good-quality miRNA molecules, which was also confirmed by gene-specific real-time PCR experiments.
650    _2
$a zvířata $7 D000818
650    12
$a stanovení celkové genové exprese $7 D020869
650    _2
$a regulace genové exprese $7 D005786
650    _2
$a lidé $7 D006801
650    _2
$a mikro RNA $x genetika $x metabolismus $7 D035683
650    _2
$a molekulární biologie $x metody $7 D008967
650    _2
$a reprodukovatelnost výsledků $7 D015203
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
655    _2
$a validační studie $7 D023361
700    1_
$a Škoda, Marek $u Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic $7 xx0214658
700    1_
$a Faustová, Markéta $u Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic $7 xx0200343
700    1_
$a Vencovský, Jiří, $u Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic $d 1953- $7 jo20000080529
700    1_
$a Novota, Peter $u Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic $7 xx0061182
773    0_
$w MED00011004 $t Folia biologica $x 0015-5500 $g Roč. 59, č. 1 (2013), s. 47-50
856    41
$u https://fb.cuni.cz/file/5671/FB2013A0006.pdf $y plný text volně přístupný
910    __
$a ABA008 $b A 970 $c 89 $y 4 $z 0
990    __
$a 20140212 $b ABA008
991    __
$a 20170623112402 $b ABA008
999    __
$a ok $b bmc $g 1013819 $s 844649
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2013 $b 59 $c 1 $d 47-50 $i 0015-5500 $m Folia biologica (Praha) $n Folia biol. (Praha) $x MED00011004
GRA    __
$a NT12452 $p MZ0
LZP    __
$b NLK138 $a Pubmed-20140212

Find record

Citation metrics

Archiving options