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Elektrochemické stanovení nízkomolekulárních antioxidantů v séru
[Electrochemical determination of low-molecular-weight antioxidants in blood serum]
A. Kračmarová, M. Pohanka
Language Czech Country Czech Republic
- MeSH
- Antioxidants * analysis MeSH
- Chemistry Techniques, Analytical methods instrumentation utilization MeSH
- Cysteine analysis blood MeSH
- Electrochemical Techniques * methods instrumentation utilization MeSH
- Glutathione analysis blood MeSH
- Plasma * MeSH
- Ascorbic Acid * analysis blood MeSH
- Uric Acid analysis blood MeSH
- Humans MeSH
- Melatonin analysis blood MeSH
- Analytic Sample Preparation Methods methods instrumentation MeSH
- Oxidative Stress MeSH
- Reactive Oxygen Species chemistry MeSH
- Tocopherols analysis blood MeSH
- Vitamin A analysis blood MeSH
- Free Radicals chemistry MeSH
- Check Tag
- Humans MeSH
The aim of the study was to establish and optimize an electrochemical method for the assay of low-molecular-weight antioxidants (LMWA). We used square-wave vol¬tammetry (at 01.1 V) on screen-printed sensors with C working electrodes. Optimum parameters were: potential step and amplitude 5 mV and frequency 1 Hz. Ascorbic acid, trolox, N-acetylcysteine and melatonin were used as standard antioxidants to test the linearity and sensitivity of the method. Limits of detection were 0.09 mmol l1 for ascorbic acid, 0.04 mmol l1 for melatonin, 0.03 mmol l1 for trolox, and 0.07 mmol l1 for N-acetylcysteine. 20 ??????l samples were used. The suitability of the method for the assay was confirmed by the standard ferric reducing ability of plasma test. Both methods correlated well (correlation coefficient 0.9778).
Electrochemical determination of low-molecular-weight antioxidants in blood serum
Literatura
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- $a The aim of the study was to establish and optimize an electrochemical method for the assay of low-molecular-weight antioxidants (LMWA). We used square-wave vol¬tammetry (at 01.1 V) on screen-printed sensors with C working electrodes. Optimum parameters were: potential step and amplitude 5 mV and frequency 1 Hz. Ascorbic acid, trolox, N-acetylcysteine and melatonin were used as standard antioxidants to test the linearity and sensitivity of the method. Limits of detection were 0.09 mmol l1 for ascorbic acid, 0.04 mmol l1 for melatonin, 0.03 mmol l1 for trolox, and 0.07 mmol l1 for N-acetylcysteine. 20 ??????l samples were used. The suitability of the method for the assay was confirmed by the standard ferric reducing ability of plasma test. Both methods correlated well (correlation coefficient 0.9778).
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