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A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to CYP3A4 response element
A. Vavrova, R. Vrzal, Z. Dvorak,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23977680
DOI
10.1002/elps.201300079
Knihovny.cz E-zdroje
- MeSH
- cytochrom P-450 CYP3A genetika metabolismus MeSH
- HeLa buňky MeSH
- lidé MeSH
- molekulární sondy genetika metabolismus MeSH
- responzivní elementy * MeSH
- retardační test metody MeSH
- steroidní receptory genetika metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The electrophoretic mobility shift assay (EMSA) is a method for the study of specific DNA–protein interactions in vitro. The pregnane X receptor (PRX) is a key xenobiotic sensor that regulates the expression of drug-metabolizing enzymes andmany other genes. Radiolabeled ³²P-DNA-probes had been used in studies of PXR-DNA interactions. There is an increasing need for nonradioactive assays, due to the health, safety and environmental issues. In the current study, we present a protocol for the nonradioactive electrophoretic mobility shift assay, allowing studying interactions between human PXR with promoter DNA sequences.
Citace poskytuje Crossref.org
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