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Preventing the mixed detergent micelle effect in two-dimensional electrophoresis on Tris-Tricine gels
H. Konečná, D. Fridrichová, G. Lochmanová, J. Bárta, Z. Zdráhal,
Electrophoresis.
2013 ;
34 (13) :
1969-71.
Language English Country Germany
Document type Journal Article, Research Support, Non-U.S. Gov't
Persistent link
https://www.medvik.cz/link/bmc14051116
- MeSH
- Electrophoresis, Gel, Two-Dimensional methods MeSH
- Detergents chemistry MeSH
- Glycine analogs & derivatives chemistry MeSH
- Micelles * MeSH
- Proteome analysis MeSH
- Proteomics methods MeSH
- Plant Proteins analysis MeSH
- Solanum tuberosum chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Application of Tris-N-[Tris(hydroxymethyl)methyl]glycine gels for 2DE is hampered by formation of mixed CHAPS-SDS micelles resulting in typical swirling pattern in the low mass range, which makes reliable quantitative and qualitative gel evaluation impossible. Modification of 2DE strip equilibration procedure prevented the direct interaction between both detergents during equilibration process, thus substantially improving gel separation.
References provided by Crossref.org
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