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Time-regulated drug delivery system based on coaxially incorporated platelet α-granules for biomedical use
M. Buzgo, R. Jakubova, A. Mickova, M. Rampichova, E. Prosecka, P. Kochova, D. Lukas, E. Amler,
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NT12156
MZ0
CEP Register
Digital library NLK
Full text - Article
Source
NLK
ProQuest Central
from 2006-06-01 to 2021-01-31
Health & Medicine (ProQuest)
from 2006-06-01 to 2021-01-31
PubMed
23198681
DOI
10.2217/nnm.12.140
Knihovny.cz E-resources
- MeSH
- Chondrocytes cytology drug effects MeSH
- Cytoplasmic Granules chemistry MeSH
- Microscopy, Confocal MeSH
- Cells, Cultured MeSH
- Drug Delivery Systems methods MeSH
- Humans MeSH
- Mesenchymal Stem Cells cytology drug effects MeSH
- Nanofibers chemistry MeSH
- Tissue Scaffolds chemistry MeSH
- Transforming Growth Factor beta1 MeSH
- Cell Survival drug effects MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
AIM: Platelet derivatives serve as an efficient source of natural growth factors. In the current study, α-granules were incorporated into coaxial nanofibers. MATERIALS & METHODS: A nanofiber scaffold containing α-granules was prepared by coaxial electrospinning. The biological potential of the nanofiber scaffold was evaluated in chondrocyte and mesenchymal stem cell cultivation studies. Additionally, the concentration of TGF-β1 was determined. RESULTs: Microscopy studies showed that intact α-granules were incorporated into the coaxial nanofibers. The cultivation tests showed that the novel scaffold stimulated viability and extracellular matrix production of chondrocytes and mesenchymal stem cells. In addition, the concentration of growth factors necessary for the induction of cell proliferation significantly decreased. CONCLUSION: The system preserved α-granule bioactivity and stimulated cell viability and chondrogenic differentiation of mesenchymal stem cells. Core/shell nanofibers incorporating α-granules are a promising system for tissue engineering, particularly cartilage engineering.
References provided by Crossref.org
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