• Je něco špatně v tomto záznamu ?

Identification and partial characterisation of new members of the Ixodes ricinus defensin family

M. Tonk, A. Cabezas-Cruz, JJ. Valdés, RO. Rego, N. Rudenko, M. Golovchenko, L. Bell-Sakyi, J. de la Fuente, L. Grubhoffer,

. 2014 ; 540 (2) : 146-52.

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc14063693

The hard-bodied tick Ixodes ricinus (castor bean tick) is the most common tick species in Europe. I. ricinus is a vector of the causative agents of diseases that affect humans and animals including tick-borne encephalitis, borreliosis, tick-borne fever and babesiosis. The innate immune system provides ticks with quite an efficient defence against some pathogenic microorganisms in the event of their penetration into the tick body or through the blood meal. Antimicrobial peptides (AMPs) constitute an important feature of the tick immune system. Defensins are a well-known class of AMPs. Members of the defensin family of proteins have been reported in several tick species. So far, only two defensins had been identified from I. ricinus. In this study, we report the identification of six novel putative defensins from I. ricinus at the genomic and transcriptional levels. At the genomic level they show differences with one being intronless, while others contain two introns. The expression pattern of these molecules in the salivary glands, midgut, ovary, Malpighian tubules, haemolymph and the tick cell line IRE/CTVM19 was determined. Some of them are tissue specific while others seem to be ubiquitous. Molecular and phylogenetic analyses show that these novel members of the I. ricinus defensin family differ phylogenetically and structurally; nevertheless, the cysteine pattern is highly conserved among the family members. Finally, antimicrobial-peptide prediction tools were used to predict putative antimicrobial activity of our defensins. They show putative antimicrobial activity mainly against Gram-positive bacteria. This study displays the diversity of the defensin family in the tick I. ricinus.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc14063693
003      
CZ-PrNML
005      
20140709121313.0
007      
ta
008      
140704s2014 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.gene.2014.03.002 $2 doi
035    __
$a (PubMed)24607035
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Tonk, Miray $u Biology Centre of the AS CR, Institute of Parasitology, Branišovská 31, 37005 České Budějovice, Czech Republic; University of South Bohemia, Faculty of Science, Branišovská 31, 37005 České Budějovice, Czech Republic. Electronic address: miraytonk@paru.cas.cz.
245    10
$a Identification and partial characterisation of new members of the Ixodes ricinus defensin family / $c M. Tonk, A. Cabezas-Cruz, JJ. Valdés, RO. Rego, N. Rudenko, M. Golovchenko, L. Bell-Sakyi, J. de la Fuente, L. Grubhoffer,
520    9_
$a The hard-bodied tick Ixodes ricinus (castor bean tick) is the most common tick species in Europe. I. ricinus is a vector of the causative agents of diseases that affect humans and animals including tick-borne encephalitis, borreliosis, tick-borne fever and babesiosis. The innate immune system provides ticks with quite an efficient defence against some pathogenic microorganisms in the event of their penetration into the tick body or through the blood meal. Antimicrobial peptides (AMPs) constitute an important feature of the tick immune system. Defensins are a well-known class of AMPs. Members of the defensin family of proteins have been reported in several tick species. So far, only two defensins had been identified from I. ricinus. In this study, we report the identification of six novel putative defensins from I. ricinus at the genomic and transcriptional levels. At the genomic level they show differences with one being intronless, while others contain two introns. The expression pattern of these molecules in the salivary glands, midgut, ovary, Malpighian tubules, haemolymph and the tick cell line IRE/CTVM19 was determined. Some of them are tissue specific while others seem to be ubiquitous. Molecular and phylogenetic analyses show that these novel members of the I. ricinus defensin family differ phylogenetically and structurally; nevertheless, the cysteine pattern is highly conserved among the family members. Finally, antimicrobial-peptide prediction tools were used to predict putative antimicrobial activity of our defensins. They show putative antimicrobial activity mainly against Gram-positive bacteria. This study displays the diversity of the defensin family in the tick I. ricinus.
650    _2
$a zvířata $7 D000818
650    _2
$a proteiny členovců $x genetika $x metabolismus $7 D060829
650    _2
$a buněčné linie $7 D002460
650    _2
$a klonování DNA $7 D003001
650    _2
$a defensiny $x genetika $x metabolismus $7 D023082
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a morčata $7 D006168
650    _2
$a klíště $x genetika $7 D018884
650    _2
$a fylogeneze $7 D010802
650    _2
$a sekvenční analýza DNA $7 D017422
650    _2
$a transkriptom $7 D059467
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Cabezas-Cruz, Alejandro $u Biology Centre of the AS CR, Institute of Parasitology, Branišovská 31, 37005 České Budějovice, Czech Republic; University of South Bohemia, Faculty of Science, Branišovská 31, 37005 České Budějovice, Czech Republic. Electronic address: cabezasalejandrocruz@gmail.com.
700    1_
$a Valdés, James J $u Biology Centre of the AS CR, Institute of Parasitology, Branišovská 31, 37005 České Budějovice, Czech Republic. Electronic address: valdjj@gmail.com.
700    1_
$a Rego, Ryan O M $u Biology Centre of the AS CR, Institute of Parasitology, Branišovská 31, 37005 České Budějovice, Czech Republic. Electronic address: ryanrego@paru.cas.cz.
700    1_
$a Rudenko, Nataliia $u Biology Centre of the AS CR, Institute of Parasitology, Branišovská 31, 37005 České Budějovice, Czech Republic. Electronic address: natasha@paru.cas.cz.
700    1_
$a Golovchenko, Maryna $u Biology Centre of the AS CR, Institute of Parasitology, Branišovská 31, 37005 České Budějovice, Czech Republic. Electronic address: marina@paru.cas.cz.
700    1_
$a Bell-Sakyi, Lesley $u The Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, UK. Electronic address: lesley.sakyi@pirbright.ac.uk.
700    1_
$a de la Fuente, José $u Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA; SaBio, Instituto de Investigación de Recursos Cinegéticos, IREC-CSIC-UCLM-JCCM, 13005 Ciudad Real, Spain. Electronic address: jose_delafuente@yahoo.com.
700    1_
$a Grubhoffer, Libor $u Biology Centre of the AS CR, Institute of Parasitology, Branišovská 31, 37005 České Budějovice, Czech Republic; University of South Bohemia, Faculty of Science, Branišovská 31, 37005 České Budějovice, Czech Republic. Electronic address: liborex@paru.cas.cz.
773    0_
$w MED00001888 $t Gene $x 1879-0038 $g Roč. 540, č. 2 (2014), s. 146-52
856    41
$u https://pubmed.ncbi.nlm.nih.gov/24607035 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20140704 $b ABA008
991    __
$a 20140709121605 $b ABA008
999    __
$a ok $b bmc $g 1031177 $s 862425
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2014 $b 540 $c 2 $d 146-52 $i 1879-0038 $m Gene $n Gene $x MED00001888
LZP    __
$a Pubmed-20140704

Najít záznam

Citační ukazatele

Možnosti archivace