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Antisense oligodeoxynucleotide-mediated gene knockdown in pollen tubes
R. Bezvoda, R. Pleskot, V. Zárský, M. Potocký,
Methods in molecular biology.
2014 ;
1080 (-) :
231-6.
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
Perzistentní odkaz
https://www.medvik.cz/link/bmc14063886
- MeSH
- antisense oligonukleotidy genetika MeSH
- fenotyp MeSH
- genový knockdown * MeSH
- pylová láčka genetika růst a vývoj MeSH
- regulace genové exprese u rostlin MeSH
- tabák genetika růst a vývoj MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Specific gene knockdown mediated by the antisense oligodeoxynucleotides (AODNs) strategy recently emerged as a rapid and effective tool for probing gene role in plant cells, particularly tip-growing pollen tubes. Here, we describe the protocol for the successful employment of AODN technique in growing tobacco pollen tubes, covering AODN design, application, and analysis of the results. We also discuss the advantages and drawbacks of this method.
Citace poskytuje Crossref.org
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