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A computational study of the glycine-rich loop of mitochondrial processing peptidase
T. Kučera, M. Otyepka, A. Matušková, A. Samad, E. Kutejová, J. Janata,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Directory of Open Access Journals
od 2006
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Public Library of Science (PLoS)
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PubMed Central
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od 2006-12-01
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od 2006-01-01
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od 2006-10-01
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od 2008-01-01
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- MeSH
- časové faktory MeSH
- glycin chemie MeSH
- katalytická doména MeSH
- metaloendopeptidasy chemie metabolismus MeSH
- podjednotky proteinů chemie metabolismus MeSH
- Saccharomyces cerevisiae enzymologie MeSH
- sekundární struktura proteinů MeSH
- simulace molekulární dynamiky MeSH
- substrátová specifita MeSH
- výpočetní biologie * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
An all atomic, non-restrained molecular dynamics (MD) simulation in explicit water was used to study in detail the structural features of the highly conserved glycine-rich loop (GRL) of the α-subunit of the yeast mitochondrial processing peptidase (MPP) and its importance for the tertiary and quaternary conformation of MPP. Wild-type and GRL-deleted MPP structures were studied using non-restrained MD simulations, both in the presence and the absence of a substrate in the peptidase active site. Targeted MD simulations were employed to study the mechanism of substrate translocation from the GRL to the active site. We demonstrate that the natural conformational flexibility of the GRL is crucial for the substrate translocation process from outside the enzyme towards the MPP active site. We show that the α-helical conformation of the substrate is important not only during its initial interaction with MPP (i.e. substrate recognition), but also later, at least during the first third of the substrate translocation trajectory. Further, we show that the substrate remains in contact with the GRL during the whole first half of the translocation trajectory and that hydrophobic interactions play a major role. Finally, we conclude that the GRL acts as a precisely balanced structural element, holding the MPP subunits in a partially closed conformation regardless the presence or absence of a substrate in the active site.
Department of Biochemistry Faculty of Science Charles University Prague Czech Republic
Institute of Microbiology Academy of Sciences of the Czech Republic Prague Czech Republic
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- $a An all atomic, non-restrained molecular dynamics (MD) simulation in explicit water was used to study in detail the structural features of the highly conserved glycine-rich loop (GRL) of the α-subunit of the yeast mitochondrial processing peptidase (MPP) and its importance for the tertiary and quaternary conformation of MPP. Wild-type and GRL-deleted MPP structures were studied using non-restrained MD simulations, both in the presence and the absence of a substrate in the peptidase active site. Targeted MD simulations were employed to study the mechanism of substrate translocation from the GRL to the active site. We demonstrate that the natural conformational flexibility of the GRL is crucial for the substrate translocation process from outside the enzyme towards the MPP active site. We show that the α-helical conformation of the substrate is important not only during its initial interaction with MPP (i.e. substrate recognition), but also later, at least during the first third of the substrate translocation trajectory. Further, we show that the substrate remains in contact with the GRL during the whole first half of the translocation trajectory and that hydrophobic interactions play a major role. Finally, we conclude that the GRL acts as a precisely balanced structural element, holding the MPP subunits in a partially closed conformation regardless the presence or absence of a substrate in the active site.
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