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Alcohols are inhibitors of Saccharomyces cerevisiae multidrug-resistance pumps Pdr5p and Snq2p
D. Gášková, J. Plášek, J. Zahumenský, I. Benešová, L. Buriánková, K. Sigler,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
ProQuest Central
od 2003-03-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2006-01-01 do 2014-12-31
Health & Medicine (ProQuest)
od 2003-03-01 do Před 1 rokem
Oxford Journals Open Access Collection
od 2001-04-01
PubMed
24028576
DOI
10.1111/1567-1364.12088
Knihovny.cz E-zdroje
- MeSH
- ABC transportéry antagonisté a inhibitory genetika metabolismus MeSH
- adenosintrifosfát metabolismus MeSH
- alkoholy farmakologie MeSH
- fungální léková rezistence genetika MeSH
- ionty metabolismus MeSH
- mikrobiální testy citlivosti MeSH
- permeabilita buněčné membrány účinky léků MeSH
- Saccharomyces cerevisiae - proteiny antagonisté a inhibitory genetika metabolismus MeSH
- Saccharomyces cerevisiae účinky léků genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The effect of alcohols on cell membrane proteins has originally been assumed to be mediated by their primary action on membrane lipid matrix. Many studies carried out later on both animal and yeast cells have revealed that ethanol and other alcohols inhibit the functions of various membrane channels, receptors and solute transport proteins, and a direct interaction of alcohols with these membrane proteins has been proposed. Using our fluorescence diS-C3 (3) diagnostic assay for multidrug-resistance pump inhibitors in a set of isogenic yeast Pdr5p and Snq2p mutants, we found that n-alcohols (from ethanol to hexanol) variously affect the activity of both pumps. Beginning with propanol, these alcohols have an inhibitory effect that increases with increasing length of the alcohol acyl chain. While ethanol does not exert any inhibitory effect at any of the concentration used (up to 3%), hexanol exerts a strong inhibition at 0.1%. The alcohol-induced inhibition of MDR pumps was detected even in cells whose membrane functional and structural integrity were not compromised. This supports a notion that the inhibitory action does not necessarily involve only changes in the lipid matrix of the membrane but may entail a direct interaction of the alcohols with the pump proteins.
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