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Comparison of chemiluminescence and flow cytometry in the estimation of reactive oxygen and nitrogen species in human semen
Nabil Aziz, Jiri Novotny, Ivana Oborna, H. Fingerova, Jana Brezinova, Magda Svobodova
Jazyk angličtina Země Spojené státy americké
Typ dokumentu srovnávací studie, hodnotící studie, práce podpořená grantem
Grantová podpora
1A8621
MZ0
CEP - Centrální evidence projektů
PubMed
20413114
Knihovny.cz E-zdroje
- MeSH
- analýza spermatu metody MeSH
- dospělí MeSH
- kyselina peroxydusitá analýza metabolismus MeSH
- lidé MeSH
- luminiscenční měření * metody MeSH
- mladý dospělý MeSH
- mužská infertilita diagnóza metabolismus MeSH
- peroxid vodíku analýza metabolismus MeSH
- počet leukocytů MeSH
- průtoková cytometrie * metody MeSH
- reaktivní formy dusíku * analýza metabolismus MeSH
- reaktivní formy kyslíku * analýza metabolismus MeSH
- sperma * chemie metabolismus MeSH
- studie případů a kontrol MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- Publikační typ
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
OBJECTIVE: To determine the relative contribution of different cell types in washed sperm to the overall intracellular production of H(2)O(2) and peroxynitrite. DESIGN: Prospective study. SETTING: University hospital. PATIENT(S): Thirty-one fertile volunteers and 166 men undergoing fertility assessment were included. INTERVENTION(S): Aliquots of sperm suspension in phosphate-buffered saline solution were used for the reactive oxygen species (ROS) detection by chemiluminescence and for the detection of H(2)O(2) and peroxynitrite by flow cytometry, with use of specific fluorescent probes, carboxy-2',7'-dichlorodihydrofluorescein diacetate dye for H(2)O(2) and dihydrorhodamine 123 for peroxynitrite. Gated analysis determined the relative contribution of spermatozoa, leukocytes, and "other round cells." MAIN OUTCOME MEASURE(S): Simultaneous estimates of global ROS production assessed by chemiluminescence assay compared with flow cytometric measurements. RESULT(S): The estimates of ROS with use of chemiluminescence positively correlated with the estimates of H(2)O(2) (r = 0.53) and peroxynitrite (r = 0.62) as assessed with flow cytometry. H(2)O(2) and peroxynitrite were measurable also in samples in which chemiluminescence did not detect measurable values. Increased production of H(2)O(2) by one cell type was associated with a relative increase in its peroxynitrite production. CONCLUSION(S): The levels of ROS production measured by chemiluminescence and flow cytometry were related. Each cell type in semen contributed differently to the global intracellular levels of H(2)O(2) and peroxynitrite. Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
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- $a Aziz, Nabil $u Gynaecology and Reproductive Medicine, Liverpool Women's Hospital and the University of Liverpool, Merseyside, United Kingdom. naziz@liv.ac.uk $7 gn_A_00010723
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- $a Comparison of chemiluminescence and flow cytometry in the estimation of reactive oxygen and nitrogen species in human semen / $c Nabil Aziz, Jiri Novotny, Ivana Oborna, H. Fingerova, Jana Brezinova, Magda Svobodova
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- $a OBJECTIVE: To determine the relative contribution of different cell types in washed sperm to the overall intracellular production of H(2)O(2) and peroxynitrite. DESIGN: Prospective study. SETTING: University hospital. PATIENT(S): Thirty-one fertile volunteers and 166 men undergoing fertility assessment were included. INTERVENTION(S): Aliquots of sperm suspension in phosphate-buffered saline solution were used for the reactive oxygen species (ROS) detection by chemiluminescence and for the detection of H(2)O(2) and peroxynitrite by flow cytometry, with use of specific fluorescent probes, carboxy-2',7'-dichlorodihydrofluorescein diacetate dye for H(2)O(2) and dihydrorhodamine 123 for peroxynitrite. Gated analysis determined the relative contribution of spermatozoa, leukocytes, and "other round cells." MAIN OUTCOME MEASURE(S): Simultaneous estimates of global ROS production assessed by chemiluminescence assay compared with flow cytometric measurements. RESULT(S): The estimates of ROS with use of chemiluminescence positively correlated with the estimates of H(2)O(2) (r = 0.53) and peroxynitrite (r = 0.62) as assessed with flow cytometry. H(2)O(2) and peroxynitrite were measurable also in samples in which chemiluminescence did not detect measurable values. Increased production of H(2)O(2) by one cell type was associated with a relative increase in its peroxynitrite production. CONCLUSION(S): The levels of ROS production measured by chemiluminescence and flow cytometry were related. Each cell type in semen contributed differently to the global intracellular levels of H(2)O(2) and peroxynitrite. Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
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- $a Novotný, Jiří $u Department of Biology, Faculty of Medicine and Dentristy, Palacky University, Olomouc, Czech Republic
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- $a Oborná, Ivana, $d 1961- $7 xx0054606 $u Department of Obstetrics and Gynaecology, Faculty of Medicine and Dentristy, Palacky University, Olomouc, Czech Republic
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- $a Fingerová, H.
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- $a Březinová, Jana $7 xx0046962 $u Department of Obstetrics and Gynaecology, Faculty of Medicine and Dentristy, Palacky University, Olomouc, Czech Republic
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- $a Svobodová, Magda $7 xx0145294 $u Department of Obstetrics and Gynaecology, Faculty of Medicine and Dentristy, Palacky University, Olomouc, Czech Republic
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- $t Fertility and sterility $x 0015-0282 $g Roč. 94, č. 7 (2010), s. 2604-2608 $w MED00001795
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