Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

CDK targeting of NBS1 promotes DNA-end resection, replication restart and homologous recombination

J Falck, JV Forment, J Coates, M Mistrik, J Lukas, J Bartek, SP Jackson

. 2012 ; 13 (6) : 561-568.

Jazyk angličtina Země Anglie, Velká Británie

Perzistentní odkaz   https://www.medvik.cz/link/bmc14072956
E-zdroje Online Plný text

NLK Free Medical Journals od 2000 do Před 1 rokem
PubMed Central od 2000
Europe PubMed Central od 2000 do Před 1 rokem
ProQuest Central od 2000-07-15 do 2013-11-30
Open Access Digital Library od 2000-07-01
Medline Complete (EBSCOhost) od 2000-07-01 do Před 1 rokem
Health & Medicine (ProQuest) od 2000-07-15 do 2013-11-30
Public Health Database (ProQuest) od 2000-07-15 do 2013-11-30
Wiley Free Content od 2000 do Před 1 rokem

Odkazy

PubMed 22565321
DOI 10.1038/embor.2012.58
Knihovny.cz E-zdroje

The conserved MRE11-RAD50-NBS1 (MRN) complex is an important sensor of DNA double-strand breaks (DSBs) and facilitates DNA repair by homologous recombination (HR) and end joining. Here, we identify NBS1 as a target of cyclin-dependent kinase (CDK) phosphorylation. We show that NBS1 serine 432 phosphorylation occurs in the S, G2 and M phases of the cell cycle and requires CDK activity. This modification stimulates MRN-dependent conversion of DSBs into structures that are substrates for repair by HR. Impairment of NBS1 phosphorylation not only negatively affects DSB repair by HR, but also prevents resumption of DNA replication after replication-fork stalling. Thus, CDK-mediated NBS1 phosphorylation defines a molecular switch that controls the choice of repair mode for DSBs.

Citace poskytuje Crossref.org

Bibliografie atd.

Literatura

000      
00000naa a2200000 a 4500
001      
bmc14072956
003      
CZ-PrNML
005      
20140925175350.0
007      
ta
008      
140925s2012 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/embor.2012.58 $2 doi
035    __
$a (PubMed)22565321
040    __
$a ABA008 $d ABA008 $e AACR2 $b cze
041    0_
$a eng
044    __
$a enk
100    1_
$a Falck, J. $u Department of Biochemistry, Wellcome Trust and Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK.
245    10
$a CDK targeting of NBS1 promotes DNA-end resection, replication restart and homologous recombination / $c J Falck, JV Forment, J Coates, M Mistrik, J Lukas, J Bartek, SP Jackson
504    __
$a Literatura
520    9_
$a The conserved MRE11-RAD50-NBS1 (MRN) complex is an important sensor of DNA double-strand breaks (DSBs) and facilitates DNA repair by homologous recombination (HR) and end joining. Here, we identify NBS1 as a target of cyclin-dependent kinase (CDK) phosphorylation. We show that NBS1 serine 432 phosphorylation occurs in the S, G2 and M phases of the cell cycle and requires CDK activity. This modification stimulates MRN-dependent conversion of DSBs into structures that are substrates for repair by HR. Impairment of NBS1 phosphorylation not only negatively affects DSB repair by HR, but also prevents resumption of DNA replication after replication-fork stalling. Thus, CDK-mediated NBS1 phosphorylation defines a molecular switch that controls the choice of repair mode for DSBs.
536    __
$c Grant Number: 092096 (United Kingdom Wellcome Trust)
536    __
$c Grant Number: 11224 (United Kingdom Cancer Research UK)
536    __
$c Grant Number: 268536 (International European Research Council)
536    __
$c Grant Number: A11224 (United Kingdom Cancer Research UK)
536    __
$c Grant Number: C6/A11224 (United Kingdom Cancer Research UK)
536    __
$c Grant Number: (United Kingdom Wellcome Trust)
590    __
$a bohemika - dle Pubmed
650    02
$a substituce aminokyselin $7 D019943
650    02
$a proteinkinasa CDC2 $x chemie $7 D016203
650    12
$a proteinkinasa CDC2 $x metabolismus $7 D016203
650    02
$a proteiny buněčného cyklu $x chemie $7 D018797
650    02
$a proteiny buněčného cyklu $x genetika $7 D018797
650    12
$a proteiny buněčného cyklu $x metabolismus $7 D018797
650    02
$a nádorové buněčné linie $7 D045744
650    02
$a dvouřetězcové zlomy DNA $7 D053903
650    12
$a štěpení DNA $7 D053837
650    02
$a oprava DNA $7 D004260
650    02
$a enzymy opravy DNA $x chemie $7 D045643
650    12
$a replikace DNA $7 D004261
650    02
$a DNA vazebné proteiny $x chemie $7 D004268
650    12
$a homologní rekombinace $7 D059765
650    02
$a lidé $7 D006801
650    02
$a mutageneze cílená $7 D016297
650    02
$a Nuclear Proteins $x ch [Chemistry]
650    02
$a jaderné proteiny $x genetika $7 D009687
650    12
$a jaderné proteiny $x metabolismus $7 D009687
650    02
$a Phosphorylation
650    02
$a Protein Processing, Post-Translational
650    02
$a Serine $x ge [Genetics]
650    02
$a Serine $x me [Metabolism]
700    1_
$a Forment, J.V.
700    1_
$a Coates, J.
700    1_
$a Mistrik, M.
700    1_
$a Lukáš, Jiří $7 xx0094305
700    1_
$a Bártek, Jiří, $d 1953- $7 xx0046271
700    1_
$a Jackson, S.P.
773    0_
$t EMBO Reports $g Roč. 13, č. 6 (2012), s. 561-568 $p EMBO Rep $x 1469-221X $w MED00006590
773    0_
$p EMBO Rep $g 13(6):561-8, 2012 Jun
910    __
$a ABA008 $y 4 $z 0
990    __
$a 20140925175817 $b ABA008
991    __
$a 20140925175817 $b ABA008
999    __
$a ok $b bmc $g 1040848 $s 871853
BAS    __
$a 3
BMC    __
$a 2012 $b 13 $c 6 $d 561-568 $i 1469-221X $m Embo reports $x MED00006590 $n EMBO Rep
LZP    __
$a NLK 2014-1/lp

Najít záznam

Citační ukazatele

Možnosti archivace