-
Something wrong with this record ?
Occurrence of microsporidia as emerging pathogens in Slovak Roma children and their impact on public health
M. Halánová, A. Valenčáková, B. Malčeková, M. Kváč, B. Sak, D. Květoňová, P. Bálent, L. Čisláková,
Language English Country Poland
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Directory of Open Access Journals
from 1998
ProQuest Central
from 1997-01-01 to 2022-01-31
Open Access Digital Library
from 1997-01-01
Nursing & Allied Health Database (ProQuest)
from 1997-01-01 to 2022-01-31
ROAD: Directory of Open Access Scholarly Resources
from 1994
PubMed
24364436
Knihovny.cz E-resources
- MeSH
- Child MeSH
- Encephalitozoon isolation & purification MeSH
- Encephalitozoonosis epidemiology ethnology MeSH
- Feces microbiology MeSH
- Infant MeSH
- Humans MeSH
- Adolescent MeSH
- Communicable Diseases, Emerging epidemiology ethnology MeSH
- Child, Preschool MeSH
- Public Health MeSH
- Check Tag
- Child MeSH
- Infant MeSH
- Humans MeSH
- Adolescent MeSH
- Male MeSH
- Child, Preschool MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Slovakia MeSH
INTRODUCTION AND OBJECTIVE: Microsporidia are identified as ubiquitous organisms of almost every animal group and are now recognized as emerging opportunistic pathogens of human. The risk factors include immunodeficiency, lack of sanitation, and exposure to contaminated water and infected animals. In Slovakia, the places with an increased risk of infection due to the presence of risk factors and routes of transmission are represented by Roma settlements. Therefore, the aim of this work was to study the occurrence of Encephalitozoon spp. and E. bieneusi in children living in Roma settlements. MATERIALS AND METHODS: Stool samples were examined of 72 clinically healthy children coming from a group of the non-integrated Roma minority for the presence of microsporidia Encephalitozoon spp. and E. bieneusi. Microsporidian spores were detected by standard Rylux D, staining and by PCR and DNA sequencing. RESULTS: Of the total number of 72 stool smears examined, 22 were positive, which represented 30.6%. By the Real Time PCR, E. bieneusi was detected in 3 samples (4.2 %) and E. cuniculi in 19 samples (26.4 %). By comparing the sequences with sequences in the GenBank, E. cuniculi genotype I (Accession No. AJ005581.1) and E. bieneusi genotype A (Accession No. AF101197.1). CONCLUSIONS: Microsporidia, as newly emerging pathogens of humans and animals, are characterised by the production of spores which are environmentally resistant. Diseases caused by them have a cosmopolitan occurrence. Although E. bieneusi and E. cuniculi belong to the most frequently diagnosed species of microsporidia in humans, in Slovakia, this is the first confirmed evidence of E. bieneusi genotype A, as well as E. cuniculi genotype I in humans by the molecular method.
- 000
- 00000naa a2200000 a 4500
- 001
- bmc14074386
- 003
- CZ-PrNML
- 005
- 20141007105225.0
- 007
- ta
- 008
- 141006s2013 pl f 000 0|eng||
- 009
- AR
- 035 __
- $a (PubMed)24364436
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a pl
- 100 1_
- $a Halánová, Monika $u Pavol Jozef Šafárik University, Faculty of Medicine, Department of Public Health, Košice, Slovak Republic.
- 245 10
- $a Occurrence of microsporidia as emerging pathogens in Slovak Roma children and their impact on public health / $c M. Halánová, A. Valenčáková, B. Malčeková, M. Kváč, B. Sak, D. Květoňová, P. Bálent, L. Čisláková,
- 520 9_
- $a INTRODUCTION AND OBJECTIVE: Microsporidia are identified as ubiquitous organisms of almost every animal group and are now recognized as emerging opportunistic pathogens of human. The risk factors include immunodeficiency, lack of sanitation, and exposure to contaminated water and infected animals. In Slovakia, the places with an increased risk of infection due to the presence of risk factors and routes of transmission are represented by Roma settlements. Therefore, the aim of this work was to study the occurrence of Encephalitozoon spp. and E. bieneusi in children living in Roma settlements. MATERIALS AND METHODS: Stool samples were examined of 72 clinically healthy children coming from a group of the non-integrated Roma minority for the presence of microsporidia Encephalitozoon spp. and E. bieneusi. Microsporidian spores were detected by standard Rylux D, staining and by PCR and DNA sequencing. RESULTS: Of the total number of 72 stool smears examined, 22 were positive, which represented 30.6%. By the Real Time PCR, E. bieneusi was detected in 3 samples (4.2 %) and E. cuniculi in 19 samples (26.4 %). By comparing the sequences with sequences in the GenBank, E. cuniculi genotype I (Accession No. AJ005581.1) and E. bieneusi genotype A (Accession No. AF101197.1). CONCLUSIONS: Microsporidia, as newly emerging pathogens of humans and animals, are characterised by the production of spores which are environmentally resistant. Diseases caused by them have a cosmopolitan occurrence. Although E. bieneusi and E. cuniculi belong to the most frequently diagnosed species of microsporidia in humans, in Slovakia, this is the first confirmed evidence of E. bieneusi genotype A, as well as E. cuniculi genotype I in humans by the molecular method.
- 650 _2
- $a mladiství $7 D000293
- 650 _2
- $a dítě $7 D002648
- 650 _2
- $a předškolní dítě $7 D002675
- 650 _2
- $a objevující se infekční nemoci $x epidemiologie $x etnologie $7 D021821
- 650 _2
- $a Encephalitozoon $x izolace a purifikace $7 D016819
- 650 _2
- $a encephalitozoonóza $x epidemiologie $x etnologie $7 D016890
- 650 _2
- $a feces $x mikrobiologie $7 D005243
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a kojenec $7 D007223
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a veřejné zdravotnictví $7 D011634
- 651 _2
- $a Slovenská republika $x epidemiologie $x etnologie $7 D018154
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Valenčáková, Alexandra $u Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic.
- 700 1_
- $a Malčeková, Beáta $u Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic.
- 700 1_
- $a Kváč, Martin $u Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, Czech Republic; Faculty of Agriculture, University of South Bohemia in České Budějovice, Czech Republic.
- 700 1_
- $a Sak, Bohumil $u Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, Czech Republic; Faculty of Agriculture, University of South Bohemia in České Budějovice, Czech Republic.
- 700 1_
- $a Květoňová, Dana $u 2University of Veterinary Medicine and Pharmacy, Department of Biology and Genetics, Komenského 73, 041 81 Košice, Slovak Republic 5 Institute of Health and Social Science, Prešov of Sv. Elizabeth, University of Health and Social Sciences Bratislava, Bratislava, The Slovak Republic.
- 700 1_
- $a Bálent, Pavol $u Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic; Institute of Health and Social Science, Prešov of Sv. Elizabeth, University of Health and Social Sciences, Bratislava, Slovak Republic.
- 700 1_
- $a Čisláková, Lýdia $u Pavol Jozef Šafárik University, Faculty of Medicine, Department of Public Health, Košice, Slovak Republic.
- 773 0_
- $w MED00172542 $t Annals of agricultural and environmental medicine : AAEM $x 1898-2263 $g Roč. 20, č. 4 (2013), s. 695-8
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/24364436 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20141006 $b ABA008
- 991 __
- $a 20141007105703 $b ABA008
- 999 __
- $a ok $b bmc $g 1042269 $s 873298
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2013 $b 20 $c 4 $d 695-8 $i 1898-2263 $m AAEM. Annals of Agricultural and Environmental Medicine $n AAEM. Ann. Agric. Environ. Med. $x MED00172542
- LZP __
- $a Pubmed-20141006
