Occurrence of microsporidia as emerging pathogens in Slovak Roma children and their impact on public health
Jazyk angličtina Země Polsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24364436
PII: 1081373
Knihovny.cz E-zdroje
- MeSH
- dítě MeSH
- Encephalitozoon izolace a purifikace MeSH
- encephalitozoonóza epidemiologie etnologie MeSH
- feces mikrobiologie MeSH
- kojenec MeSH
- lidé MeSH
- mladiství MeSH
- objevující se infekční nemoci epidemiologie etnologie MeSH
- předškolní dítě MeSH
- veřejné zdravotnictví MeSH
- Check Tag
- dítě MeSH
- kojenec MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Slovenská republika epidemiologie etnologie MeSH
INTRODUCTION AND OBJECTIVE: Microsporidia are identified as ubiquitous organisms of almost every animal group and are now recognized as emerging opportunistic pathogens of human. The risk factors include immunodeficiency, lack of sanitation, and exposure to contaminated water and infected animals. In Slovakia, the places with an increased risk of infection due to the presence of risk factors and routes of transmission are represented by Roma settlements. Therefore, the aim of this work was to study the occurrence of Encephalitozoon spp. and E. bieneusi in children living in Roma settlements. MATERIALS AND METHODS: Stool samples were examined of 72 clinically healthy children coming from a group of the non-integrated Roma minority for the presence of microsporidia Encephalitozoon spp. and E. bieneusi. Microsporidian spores were detected by standard Rylux D, staining and by PCR and DNA sequencing. RESULTS: Of the total number of 72 stool smears examined, 22 were positive, which represented 30.6%. By the Real Time PCR, E. bieneusi was detected in 3 samples (4.2 %) and E. cuniculi in 19 samples (26.4 %). By comparing the sequences with sequences in the GenBank, E. cuniculi genotype I (Accession No. AJ005581.1) and E. bieneusi genotype A (Accession No. AF101197.1). CONCLUSIONS: Microsporidia, as newly emerging pathogens of humans and animals, are characterised by the production of spores which are environmentally resistant. Diseases caused by them have a cosmopolitan occurrence. Although E. bieneusi and E. cuniculi belong to the most frequently diagnosed species of microsporidia in humans, in Slovakia, this is the first confirmed evidence of E. bieneusi genotype A, as well as E. cuniculi genotype I in humans by the molecular method.
Chronic Infections in Mammals Due to Microsporidia
Roma Health: An Overview of Communicable Diseases in Eastern and Central Europe
More than a rabbit's tale - Encephalitozoon spp. in wild mammals and birds
