SCA2 transgenic mice are thought to be a useful model of human spinocerebellar ataxia type 2. There is no effective therapy for cerebellar degenerative disorders, therefore neurotransplantation could offer hope. The aim of this work was to assess the survival and morphology of embryonic cerebellar grafts transplanted into the cerebellum of adult SCA2 mice. Four month-old homozygous SCA2 and negative control mice were treated with bilateral intracerebellar injections of an enhanced green fluorescent protein-positive embryonic cerebellar cell suspension. Graft survival and morphology were examined three months later. Graft-derived Purkinje cells and the presence of astrocytes in the graft were detected immunohistochemically. Nissl and hematoxylin-eosin techniques were used to visualize the histological structure of the graft and surrounding host tissue. Grafts survived in all experimental mice; no differences in graft structure, between SCA2 homozygous and negative mice, were found. The grafts contained numerous Purkinje cells but long distance graft-to-host axonal connections to the deep cerebellar nuclei were rarely seen. Relatively few astrocytes were found in the center of the graft. No signs of inflammation or tissue destruction were seen in the area around the grafts. Despite good graft survival and the presence of graft-derived Purkinje cells, the structure of the graft did not seem to promise any significant specific functional effects. We have shown that the graft is available for long-term experiments. Nevertheless, it would be beneficial to search for ways of enhancement of connections between the graft and host.

Biomedical Centre Faculty of Medicine in Pilsen Charles University Husova 3 Pilsen 306 05 Czech Republic

Department of Biology Faculty of Medicine in Pilsen Charles University Prague Karlovarska 48 Pilsen 301 66 Czech Republic

Department of Medical Chemistry and Biochemistry Faculty of Medicine in Pilsen Charles University Prague Karlovarska 48 Pilsen 301 66 Czech Republic

Department of Pathophysiology Faculty of Medicine in Pilsen Charles University Lidicka 1 Pilsen 301 66 Czech Republic

Laboratory of Confocal Microscopy Faculty of Science Charles University Prague Vinicna 7 Prague 2 12844 Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc14074478

003      

CZ-PrNML

005      

20170719073348.0

007      

ta

008      

141006s2014 ie f 000 0|eng||

009      

AR

024    7_

$a 10.1016/j.neulet.2013.11.020 $2 doi

035    __

$a (PubMed)24269873

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a ie

100    1_

$a Purkartova, Zdenka $u Department of Pathophysiology, Faculty of Medicine in Pilsen, Charles University, Lidicka 1, Pilsen 301 66, Czech Republic. Electronic address: zdenka.purkartova@lfp.cuni.cz.

245    10

$a Morphological analysis of embryonic cerebellar grafts in SCA2 mice / $c Z. Purkartova, J. Tuma, M. Pesta, V. Kulda, L. Hajkova, O. Sebesta, F. Vozeh, J. Cendelin,

520    9_

$a SCA2 transgenic mice are thought to be a useful model of human spinocerebellar ataxia type 2. There is no effective therapy for cerebellar degenerative disorders, therefore neurotransplantation could offer hope. The aim of this work was to assess the survival and morphology of embryonic cerebellar grafts transplanted into the cerebellum of adult SCA2 mice. Four month-old homozygous SCA2 and negative control mice were treated with bilateral intracerebellar injections of an enhanced green fluorescent protein-positive embryonic cerebellar cell suspension. Graft survival and morphology were examined three months later. Graft-derived Purkinje cells and the presence of astrocytes in the graft were detected immunohistochemically. Nissl and hematoxylin-eosin techniques were used to visualize the histological structure of the graft and surrounding host tissue. Grafts survived in all experimental mice; no differences in graft structure, between SCA2 homozygous and negative mice, were found. The grafts contained numerous Purkinje cells but long distance graft-to-host axonal connections to the deep cerebellar nuclei were rarely seen. Relatively few astrocytes were found in the center of the graft. No signs of inflammation or tissue destruction were seen in the area around the grafts. Despite good graft survival and the presence of graft-derived Purkinje cells, the structure of the graft did not seem to promise any significant specific functional effects. We have shown that the graft is available for long-term experiments. Nevertheless, it would be beneficial to search for ways of enhancement of connections between the graft and host.

650    _2

$a zvířata $7 D000818

650    _2

$a mozeček $x patologie $x transplantace $7 D002531

650    _2

$a ženské pohlaví $7 D005260

650    12

$a transplantace fetální tkáně $7 D016332

650    _2

$a přežívání štěpu $7 D006085

650    _2

$a mužské pohlaví $7 D008297

650    _2

$a myši transgenní $7 D008822

650    _2

$a sexuální faktory $7 D012737

650    _2

$a spinocerebelární ataxie $x terapie $7 D020754

655    _2

$a časopisecké články $7 D016428

655    _2

$a práce podpořená grantem $7 D013485

700    1_

$a Tuma, Jan $u Department of Pathophysiology, Faculty of Medicine in Pilsen, Charles University, Lidicka 1, Pilsen 301 66, Czech Republic; Biomedical Centre, Faculty of Medicine in Pilsen, Charles University, Husova 3, Pilsen 306 05, Czech Republic. Electronic address: jena.tuma@gmail.com.

700    1_

$a Pesta, Martin $u Department of Biology, Faculty of Medicine in Pilsen, Charles University in Prague, Karlovarska 48, Pilsen 301 66, Czech Republic. Electronic address: martin.pesta@lfp.cuni.cz.

700    1_

$a Kulda, Vlastimil $u Department of Medical Chemistry and Biochemistry, Faculty of Medicine in Pilsen, Charles University in Prague, Karlovarska 48, Pilsen 301 66, Czech Republic. Electronic address: vlastimil.kulda@lfp.cuni.cz.

700    1_

$a Hájková, Lucie, $d 1967- $7 xx0107590 $u Department of Biology, Faculty of Medicine in Pilsen, Charles University in Prague, Karlovarska 48, Pilsen 301 66, Czech Republic. Electronic address: lucie.hajkova@lfp.cuni.cz.

700    1_

$a Sebesta, Ondrej $u Laboratory of Confocal Microscopy, Faculty of Science, Charles University in Prague, Vinicna 7, Prague 2 12844, Czech Republic. Electronic address: sebesta@natur.cuni.cz.

700    1_

$a Vozeh, Frantisek $u Department of Pathophysiology, Faculty of Medicine in Pilsen, Charles University, Lidicka 1, Pilsen 301 66, Czech Republic; Biomedical Centre, Faculty of Medicine in Pilsen, Charles University, Husova 3, Pilsen 306 05, Czech Republic. Electronic address: frantisek.vozeh@lfp.cuni.cz.

700    1_

$a Cendelin, Jan $u Department of Pathophysiology, Faculty of Medicine in Pilsen, Charles University, Lidicka 1, Pilsen 301 66, Czech Republic; Biomedical Centre, Faculty of Medicine in Pilsen, Charles University, Husova 3, Pilsen 306 05, Czech Republic. Electronic address: jan.cendelin@lfp.cuni.cz.

773    0_

$w MED00003507 $t Neuroscience letters $x 1872-7972 $g Roč. 558, č. - (2014), s. 154-8

856    41

$u https://pubmed.ncbi.nlm.nih.gov/24269873 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20141006 $b ABA008

991    __

$a 20170719073839 $b ABA008

999    __

$a ok $b bmc $g 1042361 $s 873390

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2014 $b 558 $c - $d 154-8 $i 1872-7972 $m Neuroscience letters $n Neurosci. lett. $x MED00003507

LZP    __

$a Pubmed-20141006