Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

The variability of the large genomic segment of Ťahyňa orthobunyavirus and an all-atom exploration of its anti-viral drug resistance

P. Kilian, JJ. Valdes, D. Lecina-Casas, T. Chrudimský, D. Růžek,

. 2013 ; 20 (-) : 304-11.

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc14074623

Ťahyňa virus (TAHV), a member of the Bunyaviridae family (California complex), is an important but neglected human mosquito-borne pathogen. The virus genome is composed of three segments, i.e., small (S), medium (M), and large (L). Previous studies on genetic variability of viruses within the California complex were focused on S and M segments, but the L segment remains relatively unstudied. To assess the genetic variation and the relation to virus phenotype we analyzed the L segment sequences of biologically diverse TAHV strains isolated in the Czech Republic and Slovakia. Phylogenetic analysis covering all available sequences of the L segment of TAHV clearly revealed two distinguished lineages, tentatively named as "European" and "Asian". The L segment strains within the European lineage are highly conserved (identity 99.3%), whilst Asian strains are more genetically diverse (identity 97%). Based on sequence comparison with other bunyaviruses, several non-synonymous nucleotide substitutions unique for TAHV in the L segment were identified. We also identified specific residue substitutions in the endonuclease domain of TAHV compared with the La Crosse virus. Since the endonuclease domain of the La Crosse virus has been resolved, we employed an all energy landscape algorithm to analyze the ligand migration of a viral polymerase inhibitor. This allowed us to demonstrate, at the atomic level, that this viral polymerase inhibitor randomly explored the specific residue substitutions in the endonuclease domain of the TAHV L segment.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc14074623
003      
CZ-PrNML
005      
20230808121711.0
007      
ta
008      
141006s2013 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.meegid.2013.09.023 $2 doi
035    __
$a (PubMed)24090866
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Kilian, Patrik $u Faculty of Science, University of South Bohemia, Branišovská 31, CZ-37005 České Budějovice, Czech Republic; Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, Branišovská 31, CZ-37005 České Budějovice, Czech Republic.
245    14
$a The variability of the large genomic segment of Ťahyňa orthobunyavirus and an all-atom exploration of its anti-viral drug resistance / $c P. Kilian, JJ. Valdes, D. Lecina-Casas, T. Chrudimský, D. Růžek,
520    9_
$a Ťahyňa virus (TAHV), a member of the Bunyaviridae family (California complex), is an important but neglected human mosquito-borne pathogen. The virus genome is composed of three segments, i.e., small (S), medium (M), and large (L). Previous studies on genetic variability of viruses within the California complex were focused on S and M segments, but the L segment remains relatively unstudied. To assess the genetic variation and the relation to virus phenotype we analyzed the L segment sequences of biologically diverse TAHV strains isolated in the Czech Republic and Slovakia. Phylogenetic analysis covering all available sequences of the L segment of TAHV clearly revealed two distinguished lineages, tentatively named as "European" and "Asian". The L segment strains within the European lineage are highly conserved (identity 99.3%), whilst Asian strains are more genetically diverse (identity 97%). Based on sequence comparison with other bunyaviruses, several non-synonymous nucleotide substitutions unique for TAHV in the L segment were identified. We also identified specific residue substitutions in the endonuclease domain of TAHV compared with the La Crosse virus. Since the endonuclease domain of the La Crosse virus has been resolved, we employed an all energy landscape algorithm to analyze the ligand migration of a viral polymerase inhibitor. This allowed us to demonstrate, at the atomic level, that this viral polymerase inhibitor randomly explored the specific residue substitutions in the endonuclease domain of the TAHV L segment.
650    _2
$a sekvence aminokyselin $7 D000595
650    _2
$a antivirové látky $x farmakologie $7 D000998
650    _2
$a sekvence nukleotidů $7 D001483
650    _2
$a virová léková rezistence $x genetika $7 D024882
650    _2
$a viry kalifornské encefalitidy $x účinky léků $x genetika $x izolace a purifikace $7 D002141
650    _2
$a genetická variace $7 D014644
650    _2
$a genom virový $x genetika $7 D016679
650    _2
$a genotyp $7 D005838
650    _2
$a lidé $7 D006801
650    _2
$a molekulární sekvence - údaje $7 D008969
650    _2
$a fylogeneze $7 D010802
650    _2
$a RNA virová $x genetika $7 D012367
650    _2
$a sekvenční seřazení $7 D016415
650    _2
$a sekvenční analýza DNA $7 D017422
650    _2
$a virové proteiny $x genetika $7 D014764
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Valdes, James J
700    1_
$a Lecina-Casas, Daniel
700    1_
$a Chrudimský, Tomáš
700    1_
$a Růžek, Daniel, $d 1981- $7 stk2008441707
773    0_
$w MED00008609 $t Infection, genetics and evolution $x 1567-7257 $g Roč. 20, č. - (2013), s. 304-11
856    41
$u https://pubmed.ncbi.nlm.nih.gov/24090866 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20141006 $b ABA008
991    __
$a 20230808121707 $b ABA008
999    __
$a ok $b bmc $g 1042506 $s 873535
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2013 $b 20 $c - $d 304-11 $i 1567-7257 $m Infection, genetics and evolution $n Infect Genet Evol $x MED00008609
LZP    __
$a Pubmed-20141006

Najít záznam

Citační ukazatele

Nahrávání dat ...

Možnosti archivace

Nahrávání dat ...