Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

The combination of mesenchymal stem cells and a bone scaffold in the treatment of vertebral body defects

V. Vaněček, K. Klíma, A. Kohout, R. Foltán, O. Jiroušek, J. Šedý, J. Štulík, E. Syková, P. Jendelová,

. 2013 ; 22 (12) : 2777-2786.

Language English Country Germany

Document type Journal Article, Research Support, Non-U.S. Gov't

Grant support
NT13477 MZ0 CEP Register

Digital library NLK
Full text - Article
Source

E-resources Online Full text

NLK Free Medical Journals from 1997 to 2014
PubMed Central from 1997 to 2014
Europe PubMed Central from 1997 to 2014
ProQuest Central from 1997-01-01 to 1 year ago
Medline Complete (EBSCOhost) from 2000-02-01 to 1 year ago
Health & Medicine (ProQuest) from 1997-01-01 to 1 year ago

PURPOSE: Vertebral body defects represent one of the most common orthopedic challenges. In order to advance the transfer of stem cell therapies into orthopedic clinical practice, we performed this study to evaluate the safety and efficacy of a composite bioartificial graft based on a hydroxyapatite bone scaffold (CEM-OSTETIC(®)) combined with human mesenchymal stem cells (MSCs) in a rat model of vertebral body defects. METHODS: Under general isoflurane anesthesia, a defect in the body of the L2 vertebra was prepared and left to heal spontaneously (group 1), implanted with scaffold material alone (group 2), or implanted with a scaffold together with 0.5 million MSCs (group 3) or 5 million MSCs (group 4). The rats were killed 8 weeks after surgery. Histological and histomorphometrical evaluation of the implant as well as micro-CT imaging of the vertebrae were performed. RESULTS: We observed a significant effect on the formation of new bone tissue in the defect in group 4 when compared to the other groups and a reduced inflammatory reaction in both groups receiving a scaffold and MSCs. We did not detect any substantial pathological changes or tumor formation after graft implantation. CONCLUSIONS: MSCs in combination with a hydroxyapatite scaffold improved the repair of a model bone defect and might represent a safe and effective alternative in the treatment of vertebral bone defects.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc14074668
003      
CZ-PrNML
005      
20181115084732.0
007      
ta
008      
141006s2013 gw f 000 0|eng||
009      
AR
024    7_
$a 10.1007/s00586-013-2991-2 $2 doi
035    __
$a (PubMed)24013719
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Vaněček, Václav $u Institute of Experimental Medicine, Academy of Sciences of the Czech Republic (ASCR), Vídeňská 1083, 142 20, Prague 4, Czech Republic. $7 xx0246192
245    14
$a The combination of mesenchymal stem cells and a bone scaffold in the treatment of vertebral body defects / $c V. Vaněček, K. Klíma, A. Kohout, R. Foltán, O. Jiroušek, J. Šedý, J. Štulík, E. Syková, P. Jendelová,
520    9_
$a PURPOSE: Vertebral body defects represent one of the most common orthopedic challenges. In order to advance the transfer of stem cell therapies into orthopedic clinical practice, we performed this study to evaluate the safety and efficacy of a composite bioartificial graft based on a hydroxyapatite bone scaffold (CEM-OSTETIC(®)) combined with human mesenchymal stem cells (MSCs) in a rat model of vertebral body defects. METHODS: Under general isoflurane anesthesia, a defect in the body of the L2 vertebra was prepared and left to heal spontaneously (group 1), implanted with scaffold material alone (group 2), or implanted with a scaffold together with 0.5 million MSCs (group 3) or 5 million MSCs (group 4). The rats were killed 8 weeks after surgery. Histological and histomorphometrical evaluation of the implant as well as micro-CT imaging of the vertebrae were performed. RESULTS: We observed a significant effect on the formation of new bone tissue in the defect in group 4 when compared to the other groups and a reduced inflammatory reaction in both groups receiving a scaffold and MSCs. We did not detect any substantial pathological changes or tumor formation after graft implantation. CONCLUSIONS: MSCs in combination with a hydroxyapatite scaffold improved the repair of a model bone defect and might represent a safe and effective alternative in the treatment of vertebral bone defects.
650    _2
$a zvířata $7 D000818
650    _2
$a transplantace kostí $x metody $7 D016025
650    _2
$a modely nemocí na zvířatech $7 D004195
650    12
$a hydroxyapatit $7 D017886
650    _2
$a lidé $7 D006801
650    _2
$a bederní obratle $x zranění $x patologie $x radiografie $7 D008159
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a transplantace mezenchymálních kmenových buněk $x metody $7 D045164
650    _2
$a náhodné rozdělení $7 D011897
650    _2
$a krysa rodu Rattus $7 D051381
650    _2
$a potkani Wistar $7 D017208
650    _2
$a poranění páteře $x patologie $x radiografie $x terapie $7 D013124
650    _2
$a tkáňové inženýrství $x metody $7 D023822
650    12
$a tkáňové podpůrné struktury $7 D054457
650    _2
$a hojení ran $7 D014945
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Klíma, Karel, $d 1977- $7 xx0111560
700    1_
$a Kohout, Aleš $7 xx0081823
700    1_
$a Foltán, René, $d 1970- $7 xx0081950
700    1_
$a Jiroušek, Ondřej, $d 1974- $7 ntka172795
700    1_
$a Šedý, Jiří, $d 1980- $7 mzk2007411473
700    1_
$a Štulík, Jan, $d 1967- $7 nlk20030127573
700    1_
$a Syková, Eva, $d 1944- $7 jn20000710633
700    1_
$a Jendelová, Pavla
773    0_
$w MED00001666 $t European spine journal official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society $x 1432-0932 $g Roč. 22, č. 12 (2013), s. 2777-2786
856    41
$u https://pubmed.ncbi.nlm.nih.gov/24013719 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20141006 $b ABA008
991    __
$a 20181115084819 $b ABA008
999    __
$a ok $b bmc $g 1042551 $s 873580
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2013 $b 22 $c 12 $d 2777-2786 $i 1432-0932 $m European spine journal $n Eur Spine J $x MED00001666
GRA    __
$a NT13477 $p MZ0
LZP    __
$a Pubmed-20141006

Find record

Citation metrics

Loading data ...

Archiving options

Loading data ...