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The combination of mesenchymal stem cells and a bone scaffold in the treatment of vertebral body defects
V. Vaněček, K. Klíma, A. Kohout, R. Foltán, O. Jiroušek, J. Šedý, J. Štulík, E. Syková, P. Jendelová,
Language English Country Germany
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NT13477
MZ0
CEP Register
Digital library NLK
Full text - Article
Source
NLK
Free Medical Journals
from 1997 to 2014
PubMed Central
from 1997 to 2014
Europe PubMed Central
from 1997 to 2014
ProQuest Central
from 1997-01-01 to 1 year ago
Medline Complete (EBSCOhost)
from 2000-02-01 to 1 year ago
Health & Medicine (ProQuest)
from 1997-01-01 to 1 year ago
- MeSH
- Lumbar Vertebrae injuries pathology radiography MeSH
- Wound Healing MeSH
- Durapatite * MeSH
- Rats MeSH
- Humans MeSH
- Disease Models, Animal MeSH
- Random Allocation MeSH
- Spinal Injuries pathology radiography therapy MeSH
- Rats, Wistar MeSH
- Tissue Engineering methods MeSH
- Tissue Scaffolds * MeSH
- Bone Transplantation methods MeSH
- Mesenchymal Stem Cell Transplantation methods MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Humans MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
PURPOSE: Vertebral body defects represent one of the most common orthopedic challenges. In order to advance the transfer of stem cell therapies into orthopedic clinical practice, we performed this study to evaluate the safety and efficacy of a composite bioartificial graft based on a hydroxyapatite bone scaffold (CEM-OSTETIC(®)) combined with human mesenchymal stem cells (MSCs) in a rat model of vertebral body defects. METHODS: Under general isoflurane anesthesia, a defect in the body of the L2 vertebra was prepared and left to heal spontaneously (group 1), implanted with scaffold material alone (group 2), or implanted with a scaffold together with 0.5 million MSCs (group 3) or 5 million MSCs (group 4). The rats were killed 8 weeks after surgery. Histological and histomorphometrical evaluation of the implant as well as micro-CT imaging of the vertebrae were performed. RESULTS: We observed a significant effect on the formation of new bone tissue in the defect in group 4 when compared to the other groups and a reduced inflammatory reaction in both groups receiving a scaffold and MSCs. We did not detect any substantial pathological changes or tumor formation after graft implantation. CONCLUSIONS: MSCs in combination with a hydroxyapatite scaffold improved the repair of a model bone defect and might represent a safe and effective alternative in the treatment of vertebral bone defects.
References provided by Crossref.org
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