• Je něco špatně v tomto záznamu ?

Antibacterial properties of lucifensin in Lucilia sericata maggots after septic injury

I. Valachova, E. Prochazka, J. Bohova, P. Novak, P. Takac, J. Majtan,

. 2014 ; 4 (5) : 358-61.

Jazyk angličtina Země Tchaj-wan

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc14074821

OBJECTIVE: To investigate the antibacterial properties of lucifensin in maggots of Lucilia sericata after septic injury. METHODS: In our preliminary study we have shown that injuring the maggots with a needle soaked in lipopolysaccharide solution induced within 24 h lucifensin expression in the fat body and in the grease coupler of the salivary glands. It is assumed that lucifensin is secreted solely from this tissue into the haemolymph (similar to other insect defensins) and not into secreted/excreted products. We used high-performance liquid chromatography fractionation and radial diffusion assay to investigate the antibacterial properties of haemolymph extracted from larvae after septic injury. RESULTS: After septic injury, production of lucifensin in the haemolymph is increased. This led to higher antibacterial activity of such haemolymph in comparison to non-stimulated larvae. COCLUSIONS: These results suggest that beside the previously demonstrated role of lucifensin in the debridement therapy, lucifensin is simultaneously important as a part of the systematic immune response.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc14074821
003      
CZ-PrNML
005      
20210121103856.0
007      
ta
008      
141006s2014 ch f 000 0|eng||
009      
AR
024    7_
$a 10.12980/APJTB.4.2014C1134 $2 doi
035    __
$a (PubMed)25182719
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ch
100    1_
$a Valachova, Ivana $u Institute of Zoology, Slovak Academy of Sciences, Dubravska cesta 9, 845 06 Bratislava, Slovakia.
245    10
$a Antibacterial properties of lucifensin in Lucilia sericata maggots after septic injury / $c I. Valachova, E. Prochazka, J. Bohova, P. Novak, P. Takac, J. Majtan,
520    9_
$a OBJECTIVE: To investigate the antibacterial properties of lucifensin in maggots of Lucilia sericata after septic injury. METHODS: In our preliminary study we have shown that injuring the maggots with a needle soaked in lipopolysaccharide solution induced within 24 h lucifensin expression in the fat body and in the grease coupler of the salivary glands. It is assumed that lucifensin is secreted solely from this tissue into the haemolymph (similar to other insect defensins) and not into secreted/excreted products. We used high-performance liquid chromatography fractionation and radial diffusion assay to investigate the antibacterial properties of haemolymph extracted from larvae after septic injury. RESULTS: After septic injury, production of lucifensin in the haemolymph is increased. This led to higher antibacterial activity of such haemolymph in comparison to non-stimulated larvae. COCLUSIONS: These results suggest that beside the previously demonstrated role of lucifensin in the debridement therapy, lucifensin is simultaneously important as a part of the systematic immune response.
655    _2
$a časopisecké články $7 D016428
700    1_
$a Prochazka, Emanuel $u Institute of Zoology, Slovak Academy of Sciences, Dubravska cesta 9, 845 06 Bratislava, Slovakia.
700    1_
$a Bohova, Jana $u Institute of Zoology, Slovak Academy of Sciences, Dubravska cesta 9, 845 06 Bratislava, Slovakia.
700    1_
$a Novak, Petr $u Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic.
700    1_
$a Takac, Peter $u Institute of Zoology, Slovak Academy of Sciences, Dubravska cesta 9, 845 06 Bratislava, Slovakia ; Scientica s.r.o., Hybesova 33, 831 06, Bratislava, Slovakia.
700    1_
$a Majtan, Juraj $u Institute of Zoology, Slovak Academy of Sciences, Dubravska cesta 9, 845 06 Bratislava, Slovakia.
773    0_
$w MED00184779 $t Asian Pacific journal of tropical biomedicine $x 2221-1691 $g Roč. 4, č. 5 (2014), s. 358-61
856    41
$u https://pubmed.ncbi.nlm.nih.gov/25182719 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20141006 $b ABA008
991    __
$a 20210121103855 $b ABA008
999    __
$a ind $b bmc $g 1042704 $s 873733
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2014 $b 4 $c 5 $d 358-61 $i 2221-1691 $m Asian Pacific journal of tropical biomedicine $n Asian Pac J Trop Biomed $x MED00184779
LZP    __
$a Pubmed-20141006

Najít záznam

Citační ukazatele

    Možnosti archivace