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Příprava DNA s modifikovanými bázemi enzymatickou inkorporací modifikovaných nukleosid-trifosfátů
[Preparation of base-modified dna by enzymatic incorporation of modified nucleoside triphosphates]
Michal Hocek
Language Czech Country Czech Republic
Document type Research Support, Non-U.S. Gov't
- MeSH
- Biochemistry methods trends MeSH
- Molecular Diagnostic Techniques MeSH
- DNA-Directed DNA Polymerase * MeSH
- Nucleic Acids, Nucleotides, and Nucleosides * chemical synthesis MeSH
- Organic Chemistry Phenomena MeSH
- Polymerase Chain Reaction MeSH
- Chemistry Techniques, Synthetic MeSH
- Solid-Phase Synthesis Techniques * MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
Synthesis of base-modified oligonucleotides and DNA by polymerase incorporations of modified nucleoside triphosphates (dNTPs) is summarized. The crosscoupling synthesis of modified dNTPs, methods of their enzymatic incorporations, as well as their applications in bioanalysis and chemical biology are discussed.
Department of Organic Chemistry Faculty of Science Charles University Prague
Institute of Organic Chemistry and Biochemistry Academy of Sciences of the Czech Republic Prague
Preparation of base-modified dna by enzymatic incorporation of modified nucleoside triphosphates
Literatura
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